Craniula: A cranial window technique for prolonged imaging of brain surface vasculature with simultaneous adjacent intracerebral injection

被引：16
作者：
Zuluaga-Ramirez V. [1 ]
Rom S. [2 ]
Persidsky Y. [3 ]
机构：
[1] Temple University School of Medicine, Department of Pathology and Laboratory Medicine, 3500 N Broad St, MERB 880A, Philadelphia, 19140, PA
[2] Temple University School of Medicine, Department of Pathology and Laboratory Medicine, 3500 N Broad St, MERB 807, Philadelphia, 19140, PA
[3] Temple University School of Medicine, Department of Pathology and Laboratory Medicine, 3500 N Broad St, MERB 841, Philadelphia, 19140, PA
来源：
Fluids and Barriers of the CNS | / 12卷 / 1期
基金：
美国国家卫生研究院;
关键词：
2-photon microscopy; Blood Brain Barrier; Intravital microscopy; Leukocyte-endothelial interaction;
D O I：
10.1186/s12987-015-0021-y
中图分类号：
学科分类号：
摘要：
Background: Imaging of the brain surface vasculature following inflammatory insults is critical to study structural and functional changes in the living brain under normal and pathological conditions. Although there have been published reports relating to the changes that occur in the blood brain barrier (BBB) during the inflammatory process, the ability to visualize and track such changes in vivo and over time has proven to be problematic. Different techniques have been used to achieve visualization of pial vessels, but the approach has limits, which can jeopardize the well-being of the animals. Development of the cranial window technique provided a major advance in the acquisition of live images of the brain vasculature and its response to different insults and treatments. Methods: We describe in detail a protocol for delivery of a localized inflammatory insult to the mouse brain via a craniula (cranial window and adjacent cannula) and subsequent imaging of the mouse brain vasculature by intravital microscopy and two-photon laser scanning microscopy. The surgical implantation of the craniula can be completed in 30-45 min and images can be acquired immediately and for several months thereafter. The technique is minimally invasive and permits serial injections directly to the brain, thereby allowing longitudinal imaging studies. The craniula technique permits the study of structural and functional changes of the BBB following inflammatory insult and as such has wide application to neuroscience research. © 2015 Zuluaga-Ramirez et al.
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