Determination of myristicin in rat serum samples using microbore high performance liquid chromatography with column-switching

被引：0
作者：
H. S. Lee
J. H. Kim
K. Kim
K. S. Do
机构：
[1] Wonkwang University,College of Pharmacy
[2] Korea Research Institute of Chemical Technology,Toxicology Research Center
[3] Taejeon Health and Environment Research Institute,College of Agriculture & Life Sciences
[4] Seoul National University,undefined
来源：
Chromatographia | 1998年 / 48卷
关键词：
Column liquid chromatography; Microbore columns; Column-switching; Myristicin; Serum;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A microbore high-performance liquid chromatographic method with column-switching was developed for the analysis of myristicin from rat serum without prepurification. Deproteinization, fractionation, concentration and separation of analyte were carried out by appropriate switching of columns and using solvent mixtures. The method showed excellent precision, accuracy and speed with a detection limit of 10 ng mL−1 from 25 μL of serum. The total analysis time per sample was 25 min and the coefficients of variation for intra- and inter-assay were less than 1.8%.
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页码：365 / 368
页数：3
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