RNA interference in human foreskin fibroblasts within the three-dimensional collagen matrix

被引：0
作者：
Mark A. Carlson
Amy K. Prall
Jeremiah J. Gums
机构：
[1] VA Medical Center,Department of Surgery, VA Nebraska
[2] University of Nebraska Medical Center,Western Iowa Health Care System, Surgery 112
来源：
Molecular and Cellular Biochemistry | 2007年 / 306卷
关键词：
RNA interference; Fibroblast; Collagen; Extracellular matrix; Focal adhesion kinase; p53;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The technique of RNA interference (RNAi) was trialed in primary human foreskin fibroblasts, both in monolayer culture and in the fibroblast-populated collagen matrix. Knockdown of lamin A/C, p53, and FAK was possible with low-confluency (<50%) monolayer fibroblasts, a transfection vehicle concentration of 1%, and an siRNA concentration of 25–50 nM. Knockdown also was possible in the collagen matrix using similar reagent concentrations and a cellular density of one million fibroblasts per ml of matrix. Optimization of transfection conditions appeared to be important to increase knockdown efficiency. Consistent with prediction, knockdown of FAK induced apoptosis in the fibroblast-populated collagen matrix.
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页码：123 / 132
页数：9
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