Apigenin Reduces Proteasome Inhibition-Induced Neuronal Apoptosis by Suppressing the Cell Death Process

被引:0
|
作者
Arum Kim
Yoon Jeong Nam
Min Sung Lee
Yong Kyoo Shin
Dong Suep Sohn
Chung Soo Lee
机构
[1] Chung-Ang University,Department of Pharmacology, College of Medicine, and theBK21plus Skin Barrier Network Human Resources Development Team
[2] Soon Chun Hyang University Hospital,Department of Internal Medicine
[3] Chung-Ang University Hospital,Department of Thoracic and Cardiovascular Surgery
来源
Neurochemical Research | 2016年 / 41卷
关键词
Apigenin; Proteasome inhibition; Differentiated PC12 cells and SH-SY5Y cells; Apoptosis-related proteins; Protection;
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学科分类号
摘要
Impairment of proteasomal function has been shown to be implicated in neuronal cell degeneration. The compounds which have antioxidant and anti-inflammatory abilities appear to provide a neuroprotective effect. Flavone apigenin is known to exhibits antioxidant and anti-inflammatory effects. Nevertheless, the effect of apigenin on the proteasome inhibition-induced neuronal apoptosis has not been studied. Therefore, we assessed the effect of apigenin on the proteasome inhibition-induced apoptotic neuronal cell death using differentiated PC12 cells and human neuroblastoma SH-SY5Y cells. Apigenin attenuated the proteasome inhibitors (MG132 and MG115)-induced decrease in the levels of Bid and Bcl-2, increase in the levels of Bax and p53, loss of the mitochondrial transmembrane potential, release of cytochrome c, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and cell death in both cell lines. Apigenin attenuated the production of reactive oxygen species, the depletion and oxidation of glutathione, the formations of malondialdehyde and carbonyls in cell lines treated with proteasome inhibitors. The results show that apigenin appears to attenuate the proteasome inhibitor-induced apoptosis in differentiated PC12 cells and SH-SY5Y cells by suppressing the activation of the mitochondrial pathway, and of the caspase-8- and Bid-dependent pathways. The inhibitory effect of apigenin on the proteasome inhibitor-induced apoptosis appears to be attributed to the suppressive effect on the production of reactive oxygen species, the depletion and oxidation of glutathione and the formations of malondialdehyde and carbonyls.
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页码:2969 / 2980
页数:11
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