The nucleotide sequence of Saccharomyces cerevisiae chromosome IV

被引：81

作者：

Jacq C. ^{[1
]}

Alt-Mörbe J. ^{[2
]}

Andre B. ^{[3
]}

Arnold W. ^{[4
]}

Bahr A. ^{[5
]}

Ballesta J.P.G. ^{[6
]}

Bargues M. ^{[7
]}

Baron L. ^{[8
]}

Becker A. ^{[4
]}

Biteau N. ^{[8
]}

Blöcker H. ^{[9
]}

Blugeon C. ^{[1
]}

Boskovic J. ^{[6
]}

Brandt P. ^{[9
]}

Brückner M. ^{[10
]}

Buitrago M.J. ^{[11
]}

Coster F. ^{[12
]}

Delaveau T. ^{[1
]}

Del Rey F. ^{[11
]}

Dujon B. ^{[13
]}

Eide L.G. ^{[14
]}

Garcia-Cantalejo J.M. ^{[6
]}

Goffeau A. ^{[12
]}

Gomez-Peris A. ^{[15
]}

Granotier C. ^{[8
]}

Hanemann V. ^{[16
]}

Hankeln T. ^{[5
]}

Hoheisel J.D. ^{[17
]}

Jäger W. ^{[9
]}

Jimenez A. ^{[6
]}

Jonniaux J.-L. ^{[12
]}

Krämer C. ^{[5
]}

Küster H. ^{[4
]}

Laamanen P. ^{[18
]}

Legros Y. ^{[8
]}

Louis E. ^{[19
]}

Möller-Rieker S. ^{[5
]}

Monnet A. ^{[8
]}

Moro M. ^{[20
]}

Müller-Auer S. ^{[10
]}

Nußbaumer B. ^{[4
]}

Paricio N. ^{[7
]}

Paulin L. ^{[18
]}

Perea J. ^{[1
]}

Perez-Alonso M. ^{[7
]}

Perez-Ortin J.E. ^{[15
]}

Pohl T.M. ^{[21
]}

Prydz H. ^{[14
]}

Purnelle B. ^{[12
]}

Rasmussen S.W. ^{[22
]}

机构：

[1] Lab. de Genet. Moléculaire, URA 1302 du CNRS, Ecole Normale Supérieure, 75230 Paris Cedex 05

[2] Labor für DNA-Analytik, 79100 Freiburg

[3] Lab. Physiol. Cell. Genet. Levures, Campus Plaine CP244, 1050 Bruxelles, Boulevard du Triomphe

[4] Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, D-33501 Bielefeld

[5] Institut für Molekulargenetik, Universität Mainz, D-55099 Mainz

[6] Centro de Biologia Molecular, CSIC, UAM, Canto Blanco

[7] Department of Genetics, University of Valencia, Campus of Burjasot

[8] Pharmacia Biotech, Parc Technologique, 91898 Orsay Cedex, rue R. Razel

[9] GB-Genome Analysis, D-38124 Braunschweig

[10] Genotype GmbH, D-69259 Wilhelmsfeld

[11] Departemento de Microbiol. y Genet., Universidad de Salamanca, E-37007 Salamanca, Avenida del Campo Charro

[12] U. de Biochimie Physiologique, Univ. Catholique de Louvain, 1348 Louvain-la-Neuve, Place Croix du Sud

[13] U. de Genet. Molec. des Levures, Dept. de Biotechnologies, Institut Pasteur

[14] Biotechnology Centre of Oslo, 0371 Oslo

[15] Depto. de Bioquim. y Biol. Molecular, Facultad de Biologia, Universidad de Valencia

[16] Hans-Knöll-Institut, D-07745, Jena

[17] Molecular Genetic Genome Analysis, Deutsches Krebsforschungszentrum, D-69210 Heidelberg

[18] DNA Syntheses and Sequencing Lab., Institute of Biotechnology, University of Helsinki, FIN-00014, Helsinki

[19] Department of Yeast Genetics, Institute of Molecular Medecine, John Radcliffe Hospital, Headington

[20] L.N.C.I.B., Area Science Park, I-34012 Trieste

[21] GATC GmbH, 78467 Konstanz

[22] Carlsberg Laboratory, DK-2500 Copenhagen Valby

[23] AGON GmbH, D-12489 Berlin

[24] Katholieke Universiteit Leuven, Laboratory of Gene Technology, B-3001 Leuven, Willem de Croylaan

[25] Sanger Centre, Wellcome Trust Genome Campus, Hinxton

[26] Department of Biochemistry, Stanford University, Beckman Center, Stanford

来源：

Nature | 1997年 / 387卷 / Suppl 6632期

关键词：

D O I：

10.1038/387s075

中图分类号：

学科分类号：

摘要：

The complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome IV has been determined. Apart from chromosome XII, which contains the 1-2 Mb rDNA cluster, chromosome IV is the longest S. cerevisiae chromosome. It was split into three parts, which were sequenced by a consortium from the European Community, the Sanger Centre, and groups from St Louis and Stanford in the United States. The sequence of 1,531,974 base pairs contains 796 predicted or known genes, 318 (39.9%) of which have been previously identified. Of the 478 new genes, 225 (28.3%) are homologous to previously identified genes and 253 (32%) have unknown functions or correspond to spurious open reading frames (ORFs). On average there is one gene approximately every two kilobases. Superimposed on alternating regional variations in G+C composition, there is a large central domain with a lower G+C content that contains all the yeast transposon (Ty) elements and most of the tRNA genes. Chromosome IV shares with chromosomes II, V, XII, XIII and XV some long clustered duplications which partly explain its origin.

引用

页码：75 / 78

页数：3

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