HDAC4 inhibits the transcriptional activation of mda-7/IL-24 induced by Sp1

被引：0

作者：

Lina Pan

Hong Pan

Hao Jiang

Juan Du

Xiuli Wang

Baiqu Huang

Jun Lu

机构：

[1] Institute of Genetics and Cytology,

[2] Key Laboratory of Molecular Epigenetics of the Ministry of Education,undefined

[3] Northeast Normal University,undefined

[4] College of Chemistry and Life Science,undefined

[5] Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,undefined

[6] Tianjin Normal University,undefined

来源：

Cellular & Molecular Immunology | 2010年 / 7卷

关键词：

HDAC4; HDAC inhibitors; histone acetylation; mda-7/IL-24; Sp1;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Melanoma differentiation-associated gene/interleukin-24 (mda-7/IL-24) is a cytokine that can activate monocytes and T helper 2 cells. The expression of mda-7/IL-24 gradually fades with the progression of melanoma, and it is undetectable at the metastatic stage. Ectopic expression of mda-7/IL-24 selectively suppresses growth and induces apoptosis in cancer cells with little harm to normal cells. However, the transcriptional regulation of the mda-7/IL-24 gene has not been extensively studied. In this study, we show that the expression of mda-7/IL-24 was upregulated by the histone deacetylase (HDAC) inhibitors trichostatin A (TSA) and sodium butyrate (NaBu), whereas it was downregulated by HDAC4. We also found that the histone acetylation level and the binding of the transcriptional factor Sp1 to the mad-7 promoter were reduced upon HDAC4 treatment. Moreover, the HDAC inhibitor TSA induced histone hyperacetylation and stimulated Sp1 binding to the mda-7/IL-24 promoter, which in turn enhanced the expression of mda-7/IL-24. Therefore, we conclude that histone acetylation modification plays an important role in the regulation of mda-7/IL-24 and that the transcription factor Sp1 participates in this process.

引用

页码：221 / 226

页数：5

共 57 条

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