Identification of Fibrinogen-Binding Proteins of Aspergillus fumigatus Using Proteomic Approach

被引:1
作者
Santosh Kumar Upadhyay
Poonam Gautam
Hrishikesh Pandit
Yogendra Singh
Seemi Farhat Basir
Taruna Madan
机构
[1] Council for Scientific and Industrial Research,Institute of Genomics and Integrative Biology
[2] Jamia Millia Islamia,Department of Biosciences
[3] Innate Immunity,Department of Microbiology and Tumor Virology Program of the Abramson Comprehensive Cancer Center
[4] National Institute for Research in Reproductive Health (NIRRH),Proteomics Research Facility, Centre for Cellular and Molecular Biology
[5] University of Pennsylvania School of Medicine,undefined
[6] Council for Scientific and Industrial Research,undefined
[7] Innate Immunity,undefined
[8] National Institute for Research in Reproductive Health (NIRRH),undefined
来源
Mycopathologia | 2012年 / 173卷
关键词
Fibrinogen; Proteomics;
D O I
暂无
中图分类号
学科分类号
摘要
Aspergillus fumigatus, the main etiological agent for various forms of human aspergillosis, gets access to the respiratory system of human host by inhalation of airborne conidia. These conidia possibly adhere to extracellular matrix (ECM) proteins. Among the ECM proteins involved in adherence, fibrinogen is thought to be crucial. Here, we studied whether A. fumigatus three-week culture filtrate (3wcf) proteins promote binding of A. fumigatus to ECM proteins and promote fungal growth. We observed that incubation of ECM with 3wcf proteins led to dose- and time-dependent increase in adherence of conidia to the ECM. In order to identify the catalogue of fibrinogen-binding A. fumigatus proteins, we carried out fibrinogen affinity blotting using two-dimensional gel electrophoresed 3wcf proteins. A total of 15 fibrinogen-binding protein spots corresponding to 7 unique proteins were identified in 3wcf using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF-TOF). Among these, 4 proteins, namely, beta-glucosidase, alpha-mannosidase, pectate lyase A and oryzin precursor were predicted to have cell wall or extracellular localization, whereas amidase family protein and two hypothetical proteins did not display the signal sequence. This study reports seven novel fibrinogen-binding proteins of A. fumigatus, some of which could be further explored for targeting the adhesion phenomenon as antifungal strategy.
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页码:73 / 82
页数:9
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