Diagnostic value of in situ polymerase chain reaction in leprosy

被引：11

作者：

Dayal R. ^{[1
,3
]}

Singh S.P. ^{[1
]}

Mathur P.P. ^{[1
]}

Katoch V.M. ^{[2
]}

Katoch K. ^{[2
]}

Natrajan M. ^{[2
]}

机构：

[1] Department of Pediatrics, S.N. Medical College, Agra

[2] Central JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR), Agra

[3] Raja ki Mandi, Agra-282002, Opposite Kidwai Park

来源：

The Indian Journal of Pediatrics | 2005年 / 72卷 / 12期

关键词：

Histopathology; In situ Polymerase Chain Reaction; Leprosy;

D O I：

10.1007/BF02724408

中图分类号：

学科分类号：

摘要：

Objective: This prospective study was carried out to assess the diagnostic value of in situ Polymerase Chain Reaction in leprosy, particularly in enhancing the histopathological diagnosis. Method: Clinical examination of 20 patients (<16 yr) was done and skin smear for AFB was prepared. Biopsy of lesion site was taken for histopathological examination and in situ PCR testing. Results: The histopaihological examination confirmed the clinical diagnosis in 45% cases only; non-specific histopathology was reported in the remaining 55% cases. In situ PCR showed a positivity of 57.1% in early/localized form of leprosy (IIBT) and 61.5% in (BB/BL) group. When compared to histopathology examination, a significant enhancement of 15% in diagnosis was seen. With in situ PCR, the diagnosis could be confirmed in 4/11 (36.3%) cases with non-specific histopathological features, (which is common in early disease) in addition to confirmation of 8/9 (88.8%) histopathologically-confirmed tissue sections. Histopathology and in situ PCR, combined together, confirmed the diagnosis in 13/20 cases (65% of total cases). Conclusion: Thus, in situ PCR is an important diagnostic tool especially in early and doubtful cases of leprosy.

引用

页码：1043 / 1046

页数：3

共 20 条

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