Production of influenza H1N1 vaccine from MDCK cells using a novel disposable packed-bed bioreactor

被引:0
作者
Bo Sun
XiangHui Yu
Wei Kong
Shiyang Sun
Ping Yang
Changlin Zhu
Haihong Zhang
Yongge Wu
Yan Chen
Yuhua Shi
Xizhen Zhang
Chunlai Jiang
机构
[1] Jilin University,Nation Engineering Laboratory for AIDS Vaccine
[2] BCHT Biotechnology Company,undefined
来源
Applied Microbiology and Biotechnology | 2013年 / 97卷
关键词
Influenza virus; MDCK cells; Vaccine production; Bioreactor;
D O I
暂无
中图分类号
学科分类号
摘要
A process for human influenza H1N1 virus vaccine production from Madin–Darby canine kidney (MDCK) cells using a novel packed-bed bioreactor is described in this report. The mini-bioreactor was used to study the relationship between cell density and glucose consumption rate and to optimize the infection parameters of the influenza H1N1 virus (A/New Caledonia/20/99). The MDCK cell culture and virus infection were then monitored in a disposable perfusion bioreactor (AmProtein Current Perfusion Bioreactor) with proportional–integral–derivative control of pH, dissolved O2 (DO), agitation, and temperature. During 6 days of culture, the total cell number increased from 2.0 × 109 to 3.2 × 1010 cells. The maximum virus titers of 768 hemagglutinin units/100 μL and 7.8 × 107 50 % tissue culture infectious doses/mL were obtained 3 days after infection. These results demonstrate that using a disposable perfusion bioreactor for large-scale cultivation of MDCK cells, which allows for the control of DO, pH, and other conditions, is a convenient and stable platform for industrial-scale production of influenza vaccines.
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页码:1063 / 1070
页数:7
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