Combined application of 2D correlated spectroscopy and 2D nuclear Overhauser enhancement spectroscopy to the brain metabolites

The 2D NMR has great potential for routine use in organic chemistry, biochemistry and structural biology. The 2D correlated spectroscopy (COSY) and 2D nuclear Overhauser enhancement (NOE) spectroscopy are particularly powerful techniques for studies of scalar coupling connectivity and spatial connectivity. Here we present the 3-bond spatial connectivity of brain metabolites using in vitro 2D COSY and 2D NOE spectroscopy. The 2D spectra were obtained using 500 MHz NMR from solution of brain metabolites. In this study, we showed the 3-bond and spatial connectivity of brain metabolites detected by scalar coupling and dipolar NOE interaction. Our results may be helpful in promoting better understanding of the interactions in corresponding protons in each of metabolite and provide basic information for structure determination of neurochemicals.