Expression, Purification and Characterization of a Recombinant β-Glucosidase from Volvariella Volvacea

被引：0
|
作者：
Xun Li
Jianjun Pei
Guogan Wu
Weilan Shao
机构：
[1] Nanjing Normal University,The Key Laboratory of Microbial Engineering
[2] Southern Yangtze University,The Key Laboratory of Industrial Biotechnology under Ministry of Education
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
expression; β-glucosidase; purification;
D O I：
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中图分类号：
学科分类号：
摘要：
For the first time, a β-glucosidase gene from the edible straw mushroom, Volvariella volvacea V1-1, has been over-expressed in E. coli. The gene product was purified by chromatography showing a single band on SDS-PAGE. The recombinant enzyme had a molecular mass of 380 kDa with subunits of 97 kDa. The maximum activity was at pH 6.4 and 50 °C over a 5 min assay. The purified enzyme was stable from pH 5.6–8.0, had a half life of 1 h at 45 °C. The β-glucosidase had a Km of 0.2 mM for p-nitrophenyl-β-D-glucopyranoside.
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页码：1369 / 1373
页数：4
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