Expression, Purification and Characterization of a Recombinant β-Glucosidase from Volvariella Volvacea

被引：0
作者：
Xun Li
Jianjun Pei
Guogan Wu
Weilan Shao
机构：
[1] Nanjing Normal University,The Key Laboratory of Microbial Engineering
[2] Southern Yangtze University,The Key Laboratory of Industrial Biotechnology under Ministry of Education
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
expression; β-glucosidase; purification;
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学科分类号：
摘要：
For the first time, a β-glucosidase gene from the edible straw mushroom, Volvariella volvacea V1-1, has been over-expressed in E. coli. The gene product was purified by chromatography showing a single band on SDS-PAGE. The recombinant enzyme had a molecular mass of 380 kDa with subunits of 97 kDa. The maximum activity was at pH 6.4 and 50 °C over a 5 min assay. The purified enzyme was stable from pH 5.6–8.0, had a half life of 1 h at 45 °C. The β-glucosidase had a Km of 0.2 mM for p-nitrophenyl-β-D-glucopyranoside.
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页码：1369 / 1373
页数：4
相关论文
共 25 条
  • [1] Cai YJ(1998)β-Glucosidase components of the cellulolytic system of the edible straw mushroom, Enzyme Microb. Technol. 22 122-129
  • [2] Buswell JA(1996)Secretion of Curr. Genet. 29 227-233
  • [3] Chang ST(2000) β-glucosidase by J. Biol. Chem. 275 4973-4980
  • [4] Cummings C(1993)Cloning, expression, characterization, and nucleophile identification of family 3, Biotechnol. Bioeng. 41 846-853
  • [5] Fowler T(1996) β-glucosidase Curr. Genet. 30 417-422
  • [6] Dan S(1995)Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process Appl. Environ. Microbiol. 61 518-525
  • [7] Marton I(1999)Expression and secretion of the J. Biochem. 125 728-736
  • [8] Dekel M(undefined) extracellular β-glucosidase gene, undefined undefined undefined-undefined
  • [9] Bravdo BA(undefined), in undefined undefined undefined-undefined
  • [10] He SM(undefined)Cloning and characterization of a gene encoding a cell-bound, extracellular beta-glucosidase in the yeast undefined undefined undefined-undefined
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