Structure and expression analysis of early auxin-responsive Aux/IAA gene family in rice (Oryza sativa)

被引：266

作者：

Jain M. ^{[1
]}

Kaur N. ^{[1
]}

Garg R. ^{[1
]}

Thakur J.K. ^{[1
]}

Tyagi A.K. ^{[1
]}

Khurana J.P. ^{[1
]}

机构：

[1] Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, Benito Juarez Road

来源：

Functional & Integrative Genomics | 2006年 / 6卷 / 1期

关键词：

Aux/IAA; Auxin; Oryza sativa (rice); Phylogenetic analysis; Real-time PCR;

D O I：

10.1007/s10142-005-0005-0

中图分类号：

学科分类号：

摘要：

Auxin exerts pleiotropic effects on plant growth and development by regulating the expression of early auxin-responsive genes of auxin/ indoleacetic acid (Aux/IAA), small auxin-up RNA, and GH3 classes. These genes have been studied extensively in dicots like soybean and Arabidopsis. We had earlier characterized a cDNA of the first monocot member of Aux/IAA family from rice. The achievement of the large scale rice genome sequencing combined with the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encyclopedia provided us the opportunity to draw up the first comprehensive list of Aux/IAA genes in a monocot. By screening the available databases, we have identified 31 Aux/IAA genes having high sequence identity within the conserved domains I, II, III, and IV. The genomic organization as well as chromosomal location of all the Oryza sativa indoleacetic acid (OsIAA) genes is reported. The rice Aux/IAA proteins can be classified in two groups (A and B) on the basis of their phylogenetic relationship with Arabidopsis Aux/IAA proteins. An evolutionary pattern of the rice Aux/IAA genes has been discussed by analyzing their structure (exon/intron organization) and duplications. Interestingly, the duplication of rice Aux/IAA genes was found to be associated with chromosomal block duplication events in rice. The in-silico analysis has been complemented with real-time polymerase chain reaction analysis to quantify transcript levels of all Aux/IAA family members. OsIAA genes showed differential and overlapping organ-specific expression patterns in light- and dark-grown seedlings/plants. Although auxin enhanced the transcript abundance of most of the OsIAA genes, the effect was more pronounced on OsIAA9, 14, 19, 20, 24, and 31. These results provide a foundation for future studies on elucidating the precise role of rice Aux/IAA genes in early steps of auxin signal transduction. © Springer-Verlag 2005.

引用

页码：47 / 59

页数：12

共 55 条

[51]

Walker J.C., Key J.L., Isolation of cloned cDNAs to auxin-responsive poly(A) RNAs of elongating soybean hypocotyls, Proc. Natl. Acad. Sci. U. S. A., 79, pp. 7185-7189, (1982)

[52]

Wang X., Shi X., Hao B., Ge S., Luo J., Duplication and DNA segmental loss in the rice genome: Implications for diploidization, New Phytol., 165, pp. 937-946, (2005)

[53]

Worley C.K., Zenser N., Ramos J., Rouse D., Leyser O., Theologis A., Callis J., Degradation of Aux/IAA protein is essential for normal auxin signaling, Plant. J., 21, pp. 553-562, (2000)

[54]

Yamamoto K.T., Mori H., Imaseki H., cDNA cloning of indole-3-acetic acid regulated genes: Aux22 and SAUR from mung bean (Vigna radiata) hypocotyls tissue, Plant. Cell Physiol., 33, pp. 93-97, (1992)

[55]

Yu J., Hu S., Wang J., Wong GK., Li S., Liu B., Deng Y., Dai L., Zhou Y., Zhang X., Et al., A draft sequence of the rice genome (Oryza sativa L. ssp. indica.), Science, 296, pp. 79-92, (2002)

← 1 2 3 4 5 6 →