Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment

被引：0
|
作者：
Jun-Hyeog Jang
Chong-Pyoung Chung
机构：
[1] Inha University College of Medicine,Department of Biochemistry
[2] Seoul National University College of Dentistry,Intellectual Biointerface Engineering Center and Department of Periodontology
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
cell adhesion; extracellular matrix; fibroblast growth factor; fibronectin; fusion;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
引用
收藏
页码：1837 / 1840
页数：3
相关论文
共 50 条
  • [31] Recombinant human fibroblast growth factor-2 promotes nerve regeneration and functional recovery after mental nerve crush injury
    Lee, Sung Ho
    Jin, Wei-Peng
    Seo, Na Ri
    Pang, Kang-Mi
    Kim, Bongju
    Kim, Soung-Min
    Lee, Jong-Ho
    NEURAL REGENERATION RESEARCH, 2017, 12 (04) : 629 - 636
  • [32] Transforming growth factor-β1 modifies fibroblast growth factor-2 production in type II cells
    Li, CM
    Khosla, J
    Hoyle, P
    Sannes, PL
    CHEST, 2001, 120 (01) : 60S - 61S
  • [33] Fibroblast growth factor-2 mediates transforming growth factor-β action in prostate cancer reactive stroma
    Yang, F.
    Strand, D. W.
    Rowley, D. R.
    ONCOGENE, 2008, 27 (04) : 450 - 459
  • [34] Fibroblast Growth Factor-2 Attenuates Human Cardiac Fibroblast-Mediated Extracellular Matrix Remodeling
    Svystonyuk, Daniyil A.
    Ngu, Janet M.
    Mewhort, Holly E.
    Guzzardi, David G.
    Lipon, Brodie D.
    Park, Daniel S.
    Belke, Darrell D.
    Teng, Guoqi
    Fedak, Paul W.
    CIRCULATION, 2014, 130
  • [35] Fibroblast growth factor-2 mediates transforming growth factor-β action in prostate cancer reactive stroma
    F Yang
    D W Strand
    D R Rowley
    Oncogene, 2008, 27 : 450 - 459
  • [36] Biological Functions of the Low and High Molecular Weight Protein Isoforms of Fibroblast Growth Factor-2 in Cardiovascular Development and Disease
    Liao, Siyun
    Bodmer, Janet
    Pietras, Daniel
    Azhar, Mohamad
    Doetschman, Tom
    Schultz, Jo El J.
    DEVELOPMENTAL DYNAMICS, 2009, 238 (02) : 249 - 264
  • [37] Local application of recombinant human fibroblast growth factor-2 on bone repair: A dose-escalation prospective trial on patients with osteotomy
    Kawaguchi, Hiroshi
    Jingushi, Seiya
    Izumi, Toshihiro
    Fukunaga, Masao
    Matsushita, Takashi
    Nakamura, Takashi
    Mizuno, Kosaku
    Nakamura, Toshitaka
    Nakamura, Kozo
    JOURNAL OF ORTHOPAEDIC RESEARCH, 2007, 25 (04) : 480 - 487
  • [38] Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates
    Seras-Franzoso, Joaquin
    Peebo, Karl
    Garcia-Fruitos, Elena
    Vazquez, Esther
    Rinas, Ursula
    Villaverde, Antonio
    ACTA BIOMATERIALIA, 2014, 10 (03) : 1354 - 1359
  • [39] Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2
    Tsurushima, Hideo
    Marushima, Aiki
    Suzuki, Kensuke
    Oyane, Ayako
    Sogo, Yu
    Nakamura, Kazuhiro
    Matsumura, Akira
    Ito, Atsuo
    ACTA BIOMATERIALIA, 2010, 6 (07) : 2751 - 2759
  • [40] Alterations of blood vessel development by endothelial cells overexpressing fibroblast growth factor-2
    Ribatti, D
    Gualandris, A
    Belleri, M
    Massardi, L
    Nico, B
    Rusnati, M
    Dell'Era, P
    Vacca, A
    Roncali, L
    Presta, M
    JOURNAL OF PATHOLOGY, 1999, 189 (04) : 590 - 599
12345