The effects of a hydroalcoholic extract of silymarin on serum lipids profiles in streptozotocin induced diabetic rats

被引：8
作者：
Sajedianfard J. [1 ]
Behroozi Z. [1 ]
Nazifi S. [2 ]
机构：
[1] Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, 71345
[2] Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, 71345
来源：
Comparative Clinical Pathology | 2014年 / 23卷 / 3期
关键词：
Diabetes mellitus; Lipids profile; Silymarin; Streptozotocin;
D O I：
10.1007/s00580-013-1688-6
中图分类号：
学科分类号：
摘要：
Diabetes mellitus is a metabolic disease resulting from defects in insulin secretion, insulin action, or both. Diabetes produces disturbances in lipid profile and increased incidence of atherosclerosis. Lipid abnormalitiesare defined by increases in triglyceride (TG), total cholesterol (TC), very low-density lipoprotein cholesterol (VLDL-c), low-density lipoprotein cholesterol (LDL-c), and decreases in high-density lipoprotein cholesterol (HDL-c). Silymarin is a natural hepato-protector extracted from Silybum marianum. It is possible that that Silymarin might be able to modulate metabolic changes in the liver and pancreas. In the present study, fifty adult male Wistar rats were used in five groups: group I-control (0.9 % NaCl), group II-diabetic control (0.9%NaCl), group III-diabetic receiving 100 mg/kg silymarin, group IV-diabetic receiving 125 mg/kg, silymarin, and group V-diabetic receiving 250 mg/kg silymarin. All groups were dosed for 14 days via oral gavage. Diabetes was induced by single injection of streptozotocin in rats (45 mg/kg b.w., ip.). Fasting glucose level and weight were measured before injection, 3 days after injection, and on days 7 and 14 of treatment. At the end of day 14, blood samples were collected via a heart puncture after animals had been anaesthetized with ether. Lipid profile of the serum samples were analysed (TC, TG, HDL-c, LDL-c). The results showed that hydroalcoholic extract of silymarin increased the average weight and decreased glucose level in this period, and TC, TG, LDL-c and VLDL-c level experienceda dose dependent reduction; the level of HDL-c increased in a dose-dependent manner at the end of 14 days (P < 0/05). The results of the present study demonstrate that hydroalcoholic extract of silymarin has an overall beneficial effect on weight, glucose level and lipid profile in an experimental model. © 2013 Springer-Verlag London.
引用
收藏
页码：779 / 784
页数：5
相关论文
共 46 条
[1]  
Baynes J.W., Role of oxidative stress in the development of complication in diabetes, Diabetes, 40, pp. 405-412, (1991)
[2]  
Bedoya F.J., Solano F., Lucas M., N-Monomethyl-arginine and nicotinamide prevent streptozotocin-induced double strand DNA break formation in pancreatic rat islets, Experientia, 52, 4, pp. 344-347, (1996)
[3]  
Bharathi K.N., Shankar D.E., Evaluation of antioxidant and hypolipidemic activity of vedic guard, a polyherbal formulation, Int J Pharm Sci Res, 1, 10, pp. 114-127, (2010)
[4]  
Boden G., Role of fatty acids in the pathogenesis of insulin resistance and NIDDM, Diabetes, 46, 1, pp. 3-10, (1997)
[5]  
Bok S.H., Lee S.H., Park Y.B., Bae K.H., Son K.H., Jeong T.S., Choi M.S., Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-CoA reductase and acyl CoA: Cholesterol transferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoid, J Nutr, 129, pp. 1182-1185, (1999)
[6]  
Bolaffi J.L., Nagamatsu S., Harris J., Grodsky G.M., Protection by thymidine, an inhibitor of polyadenosinediphosphateribosylation, of streptozotocin inhibition of insulin secretion, Endocrinology, 120, 5, pp. 2117-2122, (1987)
[7]  
Brown M.S., Goldstein J.L., Koch's postulates for cholesterol, Cell, 71, pp. 187-188, (1992)
[8]  
Brownlee M., Biochemistry and molecular cell biology of diabetic complications, Nature, 414, 6865, pp. 813-820, (2001)
[9]  
Brownlee M., The pathobiology of diabetic complications: a unifying mechanism, Diabetes, 54, pp. 1615-1625, (2005)
[10]  
Burtis C.A., Ashwood E.R., Tietz Textbook of Clinical Chemistry, pp. 1002-1093, (1994)
12345