β-Caryophyllene Pretreatment Alleviates Focal Cerebral Ischemia-Reperfusion Injury by Activating PI3K/Akt Signaling Pathway

被引:0
作者
Qian Zhang
Ruidi An
Xiaocui Tian
Mei Yang
Minghang Li
Jie Lou
Lu Xu
Zhi Dong
机构
[1] Chongqing Medical University,Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, School of Pharmacy
来源
Neurochemical Research | 2017年 / 42卷
关键词
β-Caryophyllene; Ischemic-reperfusion injury; Neuroprotection; Apoptosis; PI3K/Akt signaling pathway;
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学科分类号
摘要
β-Caryophyllene (BCP) has been reported to be protective against focal cerebral ischemia–reperfusion (I/R) injury by its anti-oxidative and anti-inflammatory features. Recent study demonstrates that the BCP exhibits potential neuroprotection against I/R injury induced apoptosis, however, the mechanism remains unknown. Therefore, we investigate the underlying anti-apoptotic mechanism of BCP pretreatment in I/R injury. Sprague–Dawley rats (pretreated with BCP suspensions or solvent orally for 7 days) were subjected to transient Middle Cerebral Artery Occlusion (MCAO) for 90 min, followed by 24 h reperfusion. Results showed that BCP pretreatment improved the neurologic deficit score, lowered the infarct volume and decreased number of apoptotic cells in the hippocampus. Moreover, in western blot and RT-qPCR detections, BCP pretreatment down-regulated the expressions of Bax and p53, up-regulated the expression of Bcl-2, and enhanced the phosphorylation of Akt on Ser473. Blockage of PI3K activity by wortmannin not only abolished the BCP-induced decreases in infarct volume and neurologic deficit score, but also dramatically abrogated the enhancement of AKt phosphorylation. Our results suggested that BCP pre-treatment protects against I/R injury partly by suppressing apoptosis via PI3K/AKt signaling pathway activation.
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页码:1459 / 1469
页数:10
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