Simultaneous Analysis of 2- and 4-Methylimidazole in Caramel Color and Soft Drinks Using IC-PAD with Post-Column Addition of Hydroxide

被引：0

作者：

Xian-Bing Xu

Ding-Bo Liu

Yi Zhao

Shu-Juan Yu

Zhen-Gang Zhao

机构：

[1] South China University of Technology,College of Light Industry and Food Sciences

[2] Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,undefined

来源：

Food Analytical Methods | 2015年 / 8卷

关键词：

4-Methylimidazole; 2-Methylimidazole; HPCEC-PAD; Solid-phase extraction; Soft drink; Caramel color;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

High-performance cation exchange chromatography with pulsed integrated amperometric electrochemical detector (HPCEC-PAD) with post-column addition of hydroxide was first developed for simultaneous analysis of 4-methylimidazole (4-MeI) and 2-methylimidazole (2-MeI) in caramel color and soft drinks after solid-phase extraction (SPE). A CS12A cation exchange column was used to separate the targeted analytes with a perfect resolution (2.70). This method demonstrated low limit of quantification (0.05–10 mg/L) and excellent linearity with correlation of determination (R2 > 0.999 for 2-MeI and 0.997 for 4-MeI). Low concentrations of 4-MeI were found in five soft drinks, whereas those in caramel color were generally high. Additionally, the suggested method showed higher resolution and sensitivity comparing with previous reported methods for 4-MeI and 2-MeI analyses.

引用

页码：467 / 473

页数：6

共 67 条

[1]

Brusick DJ(1992)Genotoxicity hazard assessment of caramel colours III and IV Food Chem Toxicol 30 403-410

[2]

Jagannath DR(2002)Gas chromatographic–mass spectrometric quantification of 4-(5-)methylimidazole in roasted coffee after ion-pair extraction J Chromatogr A 976 285-291

[3]

Galloway SM(1992)Caramel colours—a historical introduction Food Chem Toxicol 30 351-357

[4]

Nestmann ER(1998)Determination and classification of added caramel color in adulterated acerola juice formulations J Agric Food Chem 46 1746-1753

[5]

Casal S(2011)Assessment of 4-(5-)methylimidazole in soft drinks and dark beer J Food Compos Anal 24 609-614

[6]

Fernandes JO(1997)Gas chromatographic-mass spectrometric determination of 4-(5) methylimidazole in ammonia caramel colour using ion-pair extraction and derivatization with isobutylchloroformate J Chromatogr A 786 299-308

[7]

Oliveira MBPP(1975)Quantitative determination of 4-methylimidazole as 1-acetyl derivative in caramel color by gas-liquid chromatography J Agric Food Chem 23 120-122

[8]

Ferreira MA(2013)Carcinogenic 4(5)-methylimidazole found in beverages, sauces, and caramel colors: chemical properties, analysis, and biological activities J Agric Food Chem 61 780-789

[9]

Chappel CI(2013)Formation of 4(5)-methylimidazole and its precursors, α-dicarbonyl compounds in Maillard model systems J Agric Food Chem 29 378-384

[10]

Howell JC(2006)Solid-phase extraction of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) from foods and beverages with subsequent liquid chromatographic-electrospray mass spectrometric quantification J Sep Sci 10 801-802

← 1 2 3 4 5 6 7 →