Tracking protein aggregation and mislocalization in cells with flow cytometry

被引：0
作者：
Yasmin M Ramdzan
Saskia Polling
Cheryl P Z Chia
Ivan H W Ng
Angelique R Ormsby
Nathan P Croft
Anthony W Purcell
Marie A Bogoyevitch
Dominic C H Ng
Paul A Gleeson
Danny M Hatters
机构：
[1] Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne, VIC
[2] Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC
来源：
Nature Methods | 2012年 / 9卷 / 5期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1038/nmeth.1930
中图分类号：
学科分类号：
摘要：
We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies. © 2012 Nature America, Inc. All rights reserved.
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页码：467 / 470
页数：3
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