Characterisation of a human acid-sensing ion channel (hASIC1a) endogenously expressed in HEK293 cells

被引：0

作者：

Gunthorpe M.J. ^{[1
]}

Smith G.D. ^{[1
]}

Davis J.B. ^{[1
]}

Randall A.D. ^{[1
]}

机构：

[1] Neuroscience Research, GlaxoSmithKline Pharmaceuticals, Harlow, Essex, CM19 5AW, New Frontiers Science Park (North)

来源：

Pflügers Archiv | 2001年 / 442卷 / 5期

关键词：

Acidosis; Capsaicin; Desensitisation; Nociceptor; Pain; Patch clamp; Proton-gated ion channel; Vanilloid;

D O I：

10.1007/s004240100584

中图分类号：

学科分类号：

摘要：

Acid-sensing ion channels (ASICs) are a new and expanding family of proton-gated cation (Na+/Ca2+) channels that are widely expressed in sensory neurons and the central nervous system. Their distribution suggests that they may play a critical role in the sensation of the pain that accompanies tissue acidosis and may also be important in detecting the subtle pH variations that occur during neuronal signalling. Here, using whole-cell patch-clamp electrophysiology and reverse transcriptase-polymerase chain reaction (RT-PCR), we show that HEK293 cells, a commonly used cell line for the expression and characterisation of many ion channels, functionally express an endogenous proton-gated conductance attributable to the activity of human ASIC1a. These data therefore represent the first functional characterisation of hASIC1 and have many important implications for the use of HEK293 cells as a host cell system for the study of ASICs, vanilloid receptor-1 and any other proton-gated channel. With this latter point in mind we have devised a simple desensitisation strategy to selectively remove the contribution of hASIC1a from proton-gated currents recorded from HEK293 cells expressing vanilloid receptor-1.

引用

页码：668 / 674

页数：6

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