Modeling the enzymatic hydrolysis of dilute-acid pretreated Douglas Fir

被引:0
作者
Daniel J. Schell
Mark F. Ruth
Melvin P. Tucker
机构
[1] National Renewable Energy Laboratory,
来源
Applied Biochemistry and Biotechnology | 1999年 / 77卷
关键词
Cellulose hydrolysis; softwood; ethanol; enzyme kinetics; cellulase;
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摘要
Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7–36 filter paper units [FPU]/g cellulose) and solids concentration (7–18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect even at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.
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页码:67 / 81
页数:14
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