MicroRNA-148a suppresses epithelial-to-mesenchymal transition by targeting ROCK1 in non-small cell lung cancer cells

被引：11
作者：
Jipeng Li
Yulan Song
Yiping Wang
Jianping Luo
Wanjun Yu
机构：
[1] Yinzhou People’s Hospital,Department of Clinical Laboratory
[2] Yinzhou People’s Hospital,Department of Nephrology
[3] Yinzhou People’s Hospital,Department of Respiratory Diseases
来源：
Molecular and Cellular Biochemistry | 2013年 / 380卷
关键词：
miR-148a; Epithelial-to-mesenchymal transition; NSCLC; ROCK1;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Recent studies have implied that miRNAs act as crucial modulators for epithelial-to-mesenchymal transition (EMT). We found that miR-148a is significantly downregulated in non-small cell lung cancer (NSCLC) compared to adjacent non-cancerous lung tissues, and the downregulated miR-148a was significantly associated with lymph-node metastasis. Functional assays demonstrated that miR-148a inhibited EMT in NSCLC cells. Moreover, miR-148a decreased 3′-untranslated region luciferase activity of ROCK1 and ROCK1 protein expression. Knockdown of ROCK1 reversed EMT resembling that of miR-148a overexpression. Furthermore, ROCK1 was widely upregulated in NSCLC, and its mRNA levels were inversely correlated with miR-148a expression. These findings suggest that miR-148a acts as a novel EMT suppressor in NSCLC cells, at least in part by modulation of ROCK1.
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页码：277 / 282
页数：5
相关论文
共 92 条
[1]  
Jemal A(2006)Cancer statistics, 2006 CA Cancer J Clin 56 106-130
[2]  
Siegel R(2011)Metastasis: new perspectives on an old problem Mol Cancer 10 22-866
[3]  
Ward E(2006)MicroRNA signatures in human cancers Nat Rev Cancer 6 857-677
[4]  
Murray T(2007)Impaired microRNA processing enhances cellular transformation and tumorigenesis Nat Genet 39 673-601
[5]  
Xu J(2008)The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 Nat Cell Biol 10 593-3765
[6]  
Smigal C(2011)MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1 Mol Cancer 10 99-1105
[7]  
Thun MJ(2012)miR-134 inhibits epithelial to mesenchymal transition by targeting FOXM1 in non-small cell lung cancer cells FEBS Lett 586 3761-13561
[8]  
Kraljevic Pavelic S(2012)miR-30a inhibits TGF-β1-induced epithelial-to-mesenchymal transition in heatocyte by targeting Snai1 Biochem Biophys Res Commun 417 1100-1179
[9]  
Sedic M(2008)A microRNA DNA methylation signature for human cancer metastasis Proc Natl Acad Sci USA 105 13556-7583
[10]  
Bosnjak H(2010)Altered expression of MiR-148a and MiR-152 in gastrointestinal cancers and its clinical significance J Gastrointest Surg 14 1170-1479
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