Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph

被引：0
作者：
Ji Eun Kim
Eun Jeong Kim
Won Jong Rhee
Tai Hyun Park
机构：
[1] Seoul National University,School of Chemical and Biological Engineering
来源：
Biotechnology and Bioprocess Engineering | 2005年 / 10卷
关键词：
recombinant protein; silkworm hemolymph;
D O I：
暂无
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学科分类号：
摘要：
The effect of silkworm hemolymph on the expression of recombinant protein inEscherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase inE. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1,3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.
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页码：353 / 356
页数：3
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