Field Assessment of Outcrossing from Transgenic Pea (Pisum Sativum L.) Plants

被引：0
|
作者：
Patricia L. Polowick
Albert Vandenberg
John D. Mahon
机构：
[1] Plant Biotechnology Institute,National Research Council of Canada
[2] NRCC #,Department of Agriculture, Crop Development Centre
[3] University of Saskatchewan,undefined
来源：
Transgenic Research | 2002年 / 11卷
关键词：
field trial; outcrossing; L.; transgenic plants;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The frequency of outcrossing from a transgenic line of peas into three cultivars ('Carneval', 'Montana', 'Tipu') was studied in the field in 1997 and 1999. Two dominant traits, normal leaf form and a highly-expressed β-glucuronidase (gusA) gene, were used as markers of pollen transfer. Because of heterogeneity in the commercial seed sources, leaf form alone was unreliable for assessing pollen migration into 'trap' plants. Of approximately 9000 offspring tested, only five plants scored positive for the presence of both markers. All five were located in 'trap' plots situated near the transgenic line. This represents a mean outcrossing rate of 0.07%.
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页码：515 / 519
页数：4
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