Deposition of ethyl glucuronide in WHP rat hair after chronic ethanol intake

被引：0
作者：
Anna Małkowska
Mirosław Szutowski
Wanda Dyr
机构：
[1] Department of Toxicology, Medical University of Warsaw, PL 02-097 Warszawa
[2] Department of Pharmacology and Physiology of the Nervous System, Institute of Psychiatry and Neurology, PL 02-957 Warszawa
来源：
Pharmacological Reports | 2012年 / 64卷 / 3期
关键词：
Alcohol abuse; Ethyl glucuronide; Gas chromatography-mass spectrometry; Rat hair; WHP rats;
D O I：
10.1016/S1734-1140(12)70854-X
中图分类号：
学科分类号：
摘要：
Background: This study investigated the relationship between ethanol intake in rats and the resulting level of ethyl glucuronide (EtG) in rat hair. Methods: Rats (n = 50) consumed a 10% ethanol solution for 4 weeks, then EtG was extracted from samples of their hair using a novel extraction procedure involving freezing and thawing. The EtG concentration was measured using gas chromatography and mass spectrometry. The animals voluntarily drank ethanol, with daily consumption in most rats exceeding 5 g/kg b.w. The silylated EtG was stable for at least 28 h. The limit of detection was 0.03 ng/mg, and the limit of quantification was 0.1 ng/mg. Results: Hair samples from rats that consumed ethanol had EtG levels ranging from 0.17-20.72 ng/mg in female rats and 0.15-13.72 ng/mg in males. There was a correlation between the amount of alcohol consumed and the EtG levels in hair from female (p < 0.01), but not male, rats. Conclusion: The method presented allows detection and quantification of EtG in rat hair. We also observed differences in EtG deposition in male and female rats. Copyright © 2012 by Institute of Pharmacology Polish Academy of Sciences.
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页码：586 / 593
页数：7
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