Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle

被引：0

作者：

José-Luis L. Rivero

Robert J. Talmadge

V. Reggie Edgerton

机构：

[1] Muscle Biology Laboratory,

[2] Department of Comparative and Pathological Anatomy,undefined

[3] Faculty of Veterinary Science,undefined

[4] University of Cordoba,undefined

[5] Campus Rabanales,undefined

[6] Road Madrid-Cadiz Km 396,undefined

[7] Animal Health Building,undefined

[8] E-14014 Cordoba,undefined

[9] Spain e-mail: an1lorij@uco.es Tel. 34-957-218143; Fax 34-957-218666,undefined

[10] Department of Human Nutrition,undefined

[11] Foods and Exercise,undefined

[12] Virginia Polytechnic Institute and State University Blacksburg,undefined

[13] VA 24061–0430,undefined

[14] USA,undefined

[15] Department of Physiological Sciences and Brain Research Institute,undefined

[16] University of California at Los Angeles,undefined

[17] Los Angeles,undefined

[18] USA,undefined

来源：

Histochemistry and Cell Biology | 1999年 / 111卷

关键词：

Fibre Type; Myosin Heavy Chain; Medial Gastrocnemius; Myofibrillar ATPase; Myofibrillar ATPase Activity;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Myofibrillar ATPase (mATPase), succinate dehydrogenase (SDH) and α-glycerophosphate dehydrogenase (GPD) activities and cross-sectional area (CSA) were measured in fibres of rat medial gastrocnemius muscle using quantitative histochemistry. The same fibres were typed immunohistochemically using monoclonal antibodies specific to selected myosin heavy chain (MHC) isoforms. The values of mATPase, SDH, GPD and CSA formed a continuum, but significant differences in mean values were observed among fibre types of presumed homogeneous MHC content. Type I fibres had the lowest mATPase activity, followed in rank order by type IIA<type IID/X<type IIB. Type IIA fibres had the highest SDH activity, followed in rank order by type IID/X>type I>type IIB. The mean GPD activity was consistently ranked according to fibre type such that type IIB>type IID/X >type IIA>type I. Type IIA fibres were the smallest, type IIB fibres were the largest and types I and IID/X were of intermediate size. Significant interrelationships between mATPase, SDH, GPD and CSA values were found on a fibre-to-fibre basis. Consequently, discrimination of fibres according to their MHC content was possible on the basis of their mATPase, SDH, GPD and CSA profiles. These intrafibre interrelationships suggest that the MHC isoform is associated with phenotypic differences in contractile, metabolic and size properties of muscle fibre types.

引用

页码：277 / 287

页数：10