Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

被引：0

作者：

D. Satinover

G. Vance

D. Van Dyke

S. Schwartz

机构：

[1] Department of Genetics and Center for Human Genetics,

[2] Case Western Reserve University School of Medicine and University Hospitals Cleveland,undefined

[3] Cleveland,undefined

[4] OH 44106-9959,undefined

[5] USA,undefined

[6] Department of Medical and Molecular Genetics,undefined

[7] Indiana University School of Medicine,undefined

[8] Indianapolis,undefined

[9] Ind.,undefined

[10] USA,undefined

[11] Henry Ford Hospital,undefined

[12] Detroit,undefined

[13] Mich.,undefined

[14] USA,undefined

来源：

Chromosoma | 2001年 / 110卷

关键词：

Average Level; Preliminary Analysis; Bacterial Artificial Chromosome; Cytogenetic Analysis; Artificial Chromosome;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentromeric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

引用

页码：275 / 283

页数：8