A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

被引:0
|
作者
Tamer Cebe
Karolin Yanar
Pınar Atukeren
Tuna Ozan
Aylin Irmak Kuruç
Ahmad Kunbaz
Mustafa Erinç Sitar
Murat Mengi
Mehmet Şerif Aydın
Mukaddes Eşrefoğlu
Seval Aydın
Ufuk Çakatay
机构
[1] Istanbul University,Basic Sciences, Cerrahpaşa Faculty of Medicine
[2] Istanbul University,Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine
[3] Istanbul University,Department of Physiology, Cerrahpaşa Faculty of Medicine
[4] Bezmialem Vakif University Medical Faculty,Department of Histology and Embryology
来源
AGE | 2014年 / 36卷
关键词
Myocardial aging; -Galactose; Protein oxidation; Lipid peroxidation; Antioxidant capacity; DNA oxidation;
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学科分类号
摘要
Age-related myocardial dysfunction has important implications with impaired redox homeostasis. Current study focused on investigation of redox homeostasis and histopathological changes in the myocardium of mimetically (MA), naturally aged (NA), and young control (YC) rats. Chronic d-galactose administration to young male Wistar rats (5 months old) was used to set up experimental aging models. We investigated 16 different oxidative damage biomarkers which have evaluated redox homeostasis of cellular macromolecules such as protein, lipid, and DNA. As a protein oxidation biomarker, advanced oxidation end products, protein carbonyl groups, protein-bound advanced glycation end products, dityrosine, kynurenine, and N-formylkynurenine concentrations in MA and NA rats were found to be significantly higher compared to those in YC rats. On the other hand, the levels of protein thiol groups were not significantly different between groups, whereas lipid peroxidation biomarkers such as conjugated diens, lipid hydroperoxides, and malondialdehyde in MA and NA rats were found to be significantly higher in comparison to those in YCs. For the assessment of oxidative DNA damage, we analyzed eight hydroxy-5′-deoxyguanosine concentrations of MA and NA groups which were higher than YCs. As an antioxidant status in the MA and NA groups, Cu–Zn superoxide dismutase, ferric reducing antioxidant power, and total thiol levels were lower than those in the YCs. Only nonprotein thiol levels were not significantly different. We also observed similar histopathological changes in MA and NA rats. We concluded that the mimetic aging model could be considered as a reliable experimental model for myocardial senescence.
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