Precise isolation of high-quality RNA from leaves and storage roots of cassava (Manihot esculenta Crantz) for gene expression studies

被引:0
|
作者
Jane Ijeoma Reuben-Kalu
Kokiladevi Eswaran
Raveendran Muthurajan
Uma Doraiswamy
Balasubramani Venkatasamy
Kavitha Periannagounder Shanmugam
机构
[1] National Root Crops Research Institute,Biotechnology Research and Development Center
[2] Tamil Nadu Agricultural University,Department of Plant Biotechnology, Center for Plant Molecular Biology and Biotechnology
[3] Tamil Nadu Agricultural University,Department of Biochemistry, Center for Plant Molecular Biology and Biotechnology
[4] Tamil Nadu Agricultural University,Nammazhvar Organic Farming Research Center
关键词
Cassava (; Crantz); Leaf and storage root; RNA isolation; Polyphenols; Polysaccharides; RT-qPCR; RNA sequencing;
D O I
10.1186/s42269-023-01058-1
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [31] Expression and structure of an elongation factor-1α gene (MeEF1) from cassava (Manihot esculenta Crantz)
    S. Suhandono
    J. Hughes
    K. Brown
    M.A. Hughes
    Euphytica, 2001, 120 : 49 - 58
  • [32] Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz)
    Kun Pan
    Cheng Lu
    Peixian Nie
    Meizhen Hu
    Xincheng Zhou
    Xin Chen
    Wenquan Wang
    BMC Plant Biology, 21
  • [33] Hydrogen peroxide and flavan-3-ols in storage roots of cassava (Manihot esculenta crantz) during postharvest deterioration
    Buschmann, H
    Reilly, K
    Rodriguez, MX
    Tohme, J
    Beeching, JR
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (11) : 5522 - 5529
  • [34] A Cassava vein mosaic virus promoter cassette induces high and stable gene expression in clonally propagated transgenic cassava (Manihot esculenta Crantz)
    Oyelakin, Olufemi O.
    Opabode, Jelili T.
    Raji, Adebola A.
    Ingelbrecht, Ivan L.
    SOUTH AFRICAN JOURNAL OF BOTANY, 2015, 97 : 184 - 190
  • [35] Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz)
    Ruimei Li
    Yimeng Ji
    Jie Fan
    Chenglong Yang
    Yuan Yao
    Yang Zhou
    Ruijun Duan
    Jiao Liu
    Shaoping Fu
    Xinwen Hu
    Jianchun Guo
    Acta Physiologiae Plantarum, 2014, 36 : 3089 - 3093
  • [36] Expression and structure of an elongation factor-1α gene (MeEF1) from cassava (Manihot esculenta Crantz)
    Suhandono, S
    Hughes, J
    Brown, K
    Hughes, MA
    EUPHYTICA, 2001, 120 (01) : 49 - 58
  • [37] Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root
    Carvalho, Luiz J. C. B.
    Agustini, Marco A. V.
    Anderson, James V.
    Vieira, Eduardo A.
    de Souza, Claudia R. B.
    Chen, Songbi
    Schaal, Barbara A.
    Silva, Joseane P.
    BMC PLANT BIOLOGY, 2016, 16
  • [38] Description of the puparium of Hermetia teevani Curran (Diptera, Stratiomyidae) reared from roots of cassava (Manihot esculenta Crantz)
    Pujol-Luz, Jose Roberto
    de Godoi, Fabio Siqueira Pitaluga
    de Oliveira, Charles Martins
    Barros-Cordeiro, Karine Brenda
    Mousinho, Lucas Pessanha
    ZOOTAXA, 2020, 5336 (02) : 217 - 232
  • [39] Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root
    Luiz JCB Carvalho
    Marco AV Agustini
    James V Anderson
    Eduardo A Vieira
    Claudia RB de Souza
    Songbi Chen
    Barbara A Schaal
    Joseane P Silva
    BMC Plant Biology, 16
  • [40] High Nitrogen Availability Limits Photosynthesis and Compromises Carbohydrate Allocation to Storage in Roots of Manihot esculenta Crantz
    Omondi, John Okoth
    Lazarovitch, Naftali
    Rachmilevitch, Shimon
    Yermiyahu, Uri
    Sperling, Or
    FRONTIERS IN PLANT SCIENCE, 2019, 10