Expression of p53 protein and p53 gene transcripts in rabbit carotid arteries after balloon denudation

被引：0

作者：

Masaru Aoyagi

Mari Yamamoto

Hiroshi Azuma

Goro Nagashima

Yasunari Niimi

Kimiyoshi Hirakawa

Kiyotaka Yamamoto

机构：

[1] Department of Cell Biology,

[2] Tokyo Metropolitan Institute of Gerontology,undefined

[3] 35-2 Sakae-cho,undefined

[4] Itabashi-ku,undefined

[5] Tokyo 173,undefined

[6] Japan,undefined

[7] Institute for Medical and Dental Engineering,undefined

[8] Tokyo Medical and Dental University,undefined

[9] 2-3-10 Surugadai-Kanda,undefined

[10] Chiyoda-ku,undefined

[11] Tokyo 101,undefined

[12] Japan,undefined

[13] Department of Neurosurgery,undefined

[14] School of Medicine,undefined

[15] Tokyo Medical and Dental University,undefined

[16] 1-5-45 Yushima,undefined

[17] Bunkyo-ku,undefined

[18] Tokyo 113,undefined

[19] Japan,undefined

来源：

Histochemistry and Cell Biology | 1997年 / 107卷

关键词：

Luminal; Smooth Muscle Cell; Transcript Level; Nuclear Localization; Proliferate Cell Nuclear Antigen;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Smooth muscle cell (SMC) proliferation may be positively or negatively regulated by various factors after arterial wall injury. We investigated the hypothesis that the p53 protein may play a role in regulating SMC proliferation. We examined p53 protein expression and p53 gene transcript distribution in rabbit carotid arteries over a period of 6 weeks after balloon denudation in relation to SMC proliferation. The number of p53-positive SMCs in the neointima reached a maximum 2 weeks after balloon denudation when proliferating SMCs decreased and were confined to the luminal surface of the intima. The p53-positive SMCs in the neointima almost paralleled the distribution of proliferating cell nuclear antigen (PCNA)-positive SMCs but not that of Ki-67-positive cells. Double immunofluorescence showed the simultaneous nuclear localization of both p53 protein and PCNA in intimal SMCs. Strong signals for p53 gene transcripts, identified by in situ hybridization, were observed in SMCs showing positive immunostaining for p53 protein. Our results indicate that p53 protein and p53 gene transcript levels increase, are closely linked to the proliferation of SMCs in the thickened intima, and play a key role in the regulation of cell proliferation during the repair process after arterial wall injury.

引用

页码：365 / 370

页数：5