Bioremediation of pentachlorophenol-contaminated soil by bioaugmentation using activated soil

被引：0

作者：

C. Barbeau

L. Deschênes

D. Karamanev

Y. Comeau

R. Samson

机构：

[1] NSERC Industrial Chair for Site Bioremediation,

[2] Department of Chemical Engineering,undefined

[3] Ecole Polytechnique of Montreal,undefined

[4] P.O. Box 6079,undefined

[5] Station “Centre-ville”,undefined

[6] Montreal,undefined

[7] Quebec,undefined

[8] Canada H3C 3A7 Fax: +1 514 340 5913,undefined

[9] BIOPRO Research Center,undefined

[10] Environmental Engineering Section,undefined

[11] Department of Civil Engineering,undefined

[12] Ecole Polytechnique of Montreal,undefined

[13] P.O. Box 6079,undefined

[14] Station “Centre-ville”,undefined

[15] Montreal,undefined

[16] Quebec,undefined

[17] Canada H3C 3A7,undefined

来源：

Applied Microbiology and Biotechnology | 1997年 / 48卷

关键词：

Biomass; Biodegradation; Sandy Soil; Soil Particle; Microbial Consortium;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The use of an indigenous microbial consortium, pollutant-acclimated and attached to soil particles (activated soil), was studied as a bioaugmentation method for the aerobic biodegradation of pentachlorophenol (PCP) in a contaminated soil. A 125-l completely mixed soil slurry (10% soil) bioreactor was used to produce the activated soil biomass. Results showed that the bioreactor was very effective in producing a PCP-acclimated biomass. Within 30 days, PCP-degrading bacteria increased from 105 cfu/g to 108 cfu/g soil. Mineralization of the PCP added to the reactor was demonstrated by chloride accumulation in solution. The soil-attached consortium produced in the reactor was inhibited by PCP concentrations exceeding 250 mg/l. This high level of tolerance was attributed to the beneficial effect of the soil particles. Once produced, the activated soil biomass remained active for 5 weeks at 20 °C and for up to 3 months when kept at 4 °C. The activated attached soil biomass produced in the completely mixed soil slurry bioreactor, as well as a PCP-acclimated flocculent biomass obtained from an air-lift immobilized-soil bioreactor, were used to stimulate the bioremediation of a PCP-impacted sandy soil, which had no indigenous PCP-degrading microorganisms. Bioaugmentation of this soil by the acclimated biomass resulted in a 99% reduction (from 400 mg/kg to 5 mg/kg in 130 days) in PCP concentration. The PCP degradation rates obtained with the activated soil biomass, produced either as a biomass attached to soil particles or as a flocculent biomass, were similar.

引用

页码：745 / 752

页数：7