Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis

被引：17
作者：
Jeßberger N. [1 ]
Lu Y. [1 ,2 ]
Amon J. [1 ]
Titgemeyer F. [3 ]
Sonnewald S. [4 ]
Reid S. [4 ]
Burkovski A. [1 ]
机构：
[1] Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen
[2] Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai
[3] Fachbereich Oecotrophologie, Fachhochschule Münster, Münster
[4] Lehrstuhl für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen
来源：
BMC Research Notes | / 6卷 / 1期
关键词：
AmtR; GlnR; Nitrogen control; Nitrogen metabolism; OmpR/EnvZ;
D O I：
10.1186/1756-0500-6-482
中图分类号：
学科分类号：
摘要：
Background: As other bacteria, Mycobacterium smegmatis needs adaption mechanisms to cope with changing nitrogen sources and to survive situations of nitrogen starvation. In the study presented here, transcriptome analyses were used to characterize the response of the bacterium to nitrogen starvation and to elucidate the role of specific transcriptional regulators. Results: In response to nitrogen deprivation, a general starvation response is induced in M. smegmatis. This includes changes in the transcription of several hundred genes encoding e.g. transport proteins, proteins involved in nitrogen metabolism and regulation, energy generation and protein turnover. The specific nitrogen-related changes at the transcriptional level depend mainly on the presence of GlnR, while the AmtR protein controls only a small number of genes. Conclusions: M. smegmatis is able to metabolize a number of different nitrogen sources and nitrogen control in M. smegmatis is similar to control mechanisms characterized in streptomycetes, while the master regulator of nitrogen control in corynebacteria, AmtR, is plays a minor role in this regulatory network. © 2013 Jeßberger et al.; licensee BioMed Central Ltd.
引用
收藏
相关论文
共 37 条
  • [1] Amon J., Titgemeyer F., Burkovski A., Common patterns - Unique features: Nitrogen metabolism and regulation in Gram-positive bacteria, FEMS Microbiol Rev, 34, pp. 588-605, (2010)
  • [2] Fink D., Weissschuh N., Reuther J., Wohlleben W., Engels A., Two transcriptional regulators GlnR and GlnRII are involved in regulation of nitrogen metabolism in Streptomyces coelicolor A3(2), Molecular Microbiology, 46, 2, pp. 331-347, (2002)
  • [3] Tiffert Y., Supra P., Wurm R., Wohlleben W., Wagner R., Reuther J., The Streptomyces coelicolor GlnR regulon: Identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes, Molecular Microbiology, 67, 4, pp. 861-880, (2008)
  • [4] Tiffert Y., Franz-Wachtel M., Fladerer C., Nordheim A., Reuther J., Wohlleben W., Mast Y., Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145, Appl Microbiol Biotechnol, 89, pp. 1149-1159, (2011)
  • [5] Reuther J., Wohlleben W., Nitrogen metabolism in Streptomyces coelicolor: Transcriptional and post-translational regulation, Journal of Molecular Microbiology and Biotechnology, 12, 1-2, pp. 139-146, (2007)
  • [6] Jakoby M., Nolden L., Meier-Wagner J., Kramer R., Burkovski A., AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum, Molecular Microbiology, 37, 4, pp. 964-977, (2000)
  • [7] Nolden L., Beckers G., Burkovski A., Nitrogen assimilation in Corynebacterium diphtheriae: Pathways and regulatory cascades, FEMS Microbiology Letters, 208, 2, pp. 287-293, (2002)
  • [8] Walter B., Hanssler E., Kalinowski J., Burkovski A., Nitrogen metabolism and nitrogen control in corynebacteria: Variations of a common theme, Journal of Molecular Microbiology and Biotechnology, 12, 1-2, pp. 131-138, (2007)
  • [9] Beckers G., Strosser J., Hildebrandt U., Kalinowski J., Farwick M., Kramer R., Burkovski A., Regulation of AmtR-controlled gene expression in Corynebacterium glutamicum: Mechanism and characterization of the AmtR regulon, Molecular Microbiology, 58, 2, pp. 580-595, (2005)
  • [10] Buchinger S., Strosser J., Rehm N., Hanssler E., Hans S., Bathe B., Schomburg D., Kramer R., Burkovski A., A combination of metabolome and transcriptome analyses reveals new targets of the Corynebacterium glutamicum nitrogen regulator AmtR, J Biotechnol, 140, pp. 68-74, (2009)
1234