Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

被引：0
作者：
Svetoslav Nanev Slavov
Katia Kaori Otaguiri
Dimas Tadeu Covas
Simone Kashima
机构：
[1] University of São Paulo,Laboratory of Molecular Biology, Faculty of Medicine of Ribeirão Preto, Regional Blood Center of Ribeirão Preto
[2] University of São Paulo,Department of Clinical Medicine, Faculty of Medicine of Ribeirão Preto
[3] University of São Paulo,Department of Clinical, Toxicological and Bromatological Analyses, School of Pharmaceutical Sciences
来源：
Indian Journal of Hematology and Blood Transfusion | 2016年 / 32卷
关键词：
B19V; Viral quantitation; Blood donors;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan® real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection.
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页码：323 / 325
页数：2
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