Second harmonic generation microscopy: a powerful tool for bio-imaging

被引:0
作者
Arash Aghigh
Stéphane Bancelin
Maxime Rivard
Maxime Pinsard
Heide Ibrahim
François Légaré
机构
[1] Institut National de La Recherche Scientifique,Centre Énergie Matériaux Télécommunications
[2] Univ. Bordeaux,undefined
[3] CNRS,undefined
[4] IINS,undefined
[5] UMR 5297,undefined
[6] National Research Council Canada,undefined
[7] Institut National de Recherche en Sciences Et Technologies Pour L’environnement Et L’agriculture,undefined
来源
Biophysical Reviews | 2023年 / 15卷
关键词
Non-linear microscopy; SHG; Neuroimaging; Interferometry; Polarimetry;
D O I
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中图分类号
学科分类号
摘要
Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique.
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页码:43 / 70
页数:27
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