Random amplified polymorphic DNA (RAPDS) markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L.

被引:43
作者
K. Kanwar
K. Bindiya
机构
[1] Dr. Y.S. Parmar Univ. Hort./Forest., Nauni
关键词
Cluster analysis; Genetic stability; Micropropagation; RAPDs; Somaclonal variation; Tissue-culture;
D O I
10.1023/A:1024623517374
中图分类号
学科分类号
摘要
Four years' old micropropagated plants regenerated by enhanced axillary branching from shoot buds of a single genotype of Robinia pseudoacacia were characterized by RAPDs. Random amplified polymorphic DNA analysis was carried out using 19 random 10-mer DNA primers and 286 RAPD bands were examined which showed 30% polymorphism. Similarity indices ranged from 0.86 to 0.96 among different plants based on RAPD data. The UPGMA dendrogram was constructed based on similarity indices which showed clustering of different plants into subgroups based on similarity values. Our results suggest that somaclonal DNA sequence variations are present even when organized cultures such as shoot buds were used as explant for micro-propagation.
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页码:41 / 47
页数:6
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共 41 条
[1]  
Ahuja M.R., In vitro propagation of poplar and aspen, Cell and Tissue Culture in Forestry, 3, pp. 207-223, (1987)
[2]  
Antonetti P.L.E., Pinon J., Somaclonal variation in poplar, Plant Cell Tissue and Organ Cult, 35, pp. 99-106, (1993)
[3]  
Barrett C., Lefort F., Douglas G.C., Genetic characterization of oak seedlings, epicormic, crown and micropropagated shoots from mature trees by RAPD and microsatellite PCR, Scientia Horticulturae, 70, pp. 319-330, (1997)
[4]  
Brewbaker J.L., Robinia pseudoacacia Historically and currently important. Nitrogen fixing tree, Assoc NFT Highlights, 86, (1986)
[5]  
Chalupa V., In vitro propagation of Willows. European mountain ash and Black locus, Biol Plantarum, 26, pp. 374-377, (1983)
[6]  
D'Amato F., Chromosome number variation in cultured cells and regenerated plants, Frontiers of Plant Tissue Culture, pp. 287-295, (1978)
[7]  
De Laia M.L., Comes E.A., Esbrisse E.J., Araujo E.F., Random Amplified Polymorphic DNA (RAPD) analysis of genotypic identities in Eucalylptus clones, Silvae Genetica, 49, pp. 239-243, (2000)
[8]  
Dice L.R., Measures of the amount of ecological association between species, Ecology, 26, pp. 297-302, (1945)
[9]  
Doyle J.J., Doyle J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochem Bull, 19, pp. 11-15, (1987)
[10]  
Evans D.A., Sharp W.R., Medina-Filho H.P., Somaclonal and gametoclonal variation, Amer J Bot, 77, pp. 759-774, (1984)