Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis

被引：40
作者：
Deshpande P.S. [1 ]
Kashyap R.S. [1 ]
Ramteke S.S. [1 ]
Nagdev K.J. [1 ]
Purohit H.J. [2 ]
Taori G.M. [1 ]
Daginawala H.F. [1 ]
机构：
[1] Biochemistry Research Laboratory, Central India Institute of Medical Sciences
[2] Environmental Genomics Unit, National Enviromental Engineering Research Institute, Nehru Marg
来源：
Cerebrospinal Fluid Research | / 4卷 / 1期
关键词：
Polymerase Chain Reaction Assay; Tuberculous Meningitis; Viral Meningitis; Meningeal Irritation; Pyogenic Meningitis;
D O I：
10.1186/1743-8454-4-10
中图分类号：
学科分类号：
摘要：
Background: Tuberculous meningitis (TBM) is one of the common clinical manifestations of extra-pulmonary tuberculosis. It is difficult to diagnose due to a lack of rapid, sensitive, and specific tests. Newer methods, which are easy and reliable, are required to diagnose TBM at an early stage. Thus our aim was to evaluate the polymerase chain reaction (PCR) technique, using primers directed against the IS6110 gene, for the detection of Mycobacterium tuberculosis in the CSF, for the diagnosis of TBM patients. Methods: An in-house IS6110 PCR method using a specific pair of primers designed to amplify the insertion sequence, IS6110, in the M. tuberculosis genome was used to analyze CSF. A total of 80 CSF samples from different groups of patients were studied (confirmed TBM n = 35, clinically suspected TBM n = 16, non-TBM infectious meningitis n = 12, non infectious neurological diseases n = 17). Results: PCR gave a sensitivity of 91.4% and specificity of 75.9% for the diagnosis of TBM in patients with TBM confirmed by culture. In 16 clinically diagnosed, but unconfirmed, TBM cases PCR was positive in 10 (62.5%) cases. There were seven (24.1%) PCR-positive cases among the 29 patients with non-TBM and non-infectious neurological disease. Conclusion: We conclude that the performance of an in-house IS6110 PCR assay is valuable in the rapid diagnosis of tuberculous meningitis. © 2007 Deshpande et al; licensee BioMed Central Ltd.
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