Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis

被引：0
作者：
Xue-Ming Tang
F.M. Lakay
Wei Shen
Wei-Lan Shao
Hui-Ying Fang
B.A. Prior
Zheng-Xiang Wang
Jian Zhuge
机构：
[1] Southern Yangtze University,The Key Laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microbiology
[2] University of Stellenbosch,Department of Microbiology
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
alkaline protease; characterisation; purification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60°C (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition.
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页码：1421 / 1424
页数：3
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