A study of the regenerative potential of bone marrow cells of donor mice that carry the egfp gene in irradiated mice

被引：0

作者：

Sergievich L.A. ^{[1
]}

Karnaukhova E.V. ^{[1
]}

Karnaukhov A.V. ^{[1
]}

Karnaukhova N.A. ^{[1
]}

Bogdanenko E.V. ^{[2
]}

Lizunova I.A. ^{[1
]}

Karnaukhov V.N. ^{[1
]}

机构：

[1] Institute of Cell Biophysics, Russian Academy of Sciences, Moscow oblast, Pushchino

[2] Institute of General Pathology and Pathophysiology, Moscow

来源：

Biophysics | 2018年 / 63卷 / 1期

关键词：

Bone marrow; GFP [!sup]+[!/sup] mice; Information hypothesis of aging; Irradiation; Life extension; Transplantation;

D O I：

10.1134/S0006350918010049

中图分类号：

学科分类号：

摘要：

A study of the regenerative potential of bone marrow cells of donor mice that express the enhanced green fluorescent protein was conducted in mice irradiated at a dose of 7 Gy. Expression of this protein allowed us to carry out monitoring of the presence of donor cells in recipient blood over the entire lifespan of the recipient. The lifespan of young recipients increased by 93% after transplantation; for old recipients it increased by 15%. Total acceptance of the bone marrow, spleen, thymus, and blood of the recipient with donor bone marrow cells was demonstrated over the entire life of the recipient. Only the donor colonies were detected with the studied irradiation dose and number of transplanted cells (11.7 ± 0.4) · 10 6 on the spleen surface. The percentage of bone marrow and spleen cells that expressed the CD117 and CD34 stem cell markers in the recipient mice was above the control level for a long period of time after the irradiation. More than half of the cells with CD117, CD34, CD90.2, and CD45R/B220 phenotypes in the studied organs were donor cells. Further detailed study of the peculiarities of the engraftment of bone marrow cells, both without preliminary treatment of recipients and after the effects of extreme factors, will allow improvement of the methods of cell therapy. © L.A. Sergievich, E.V. Karnaukhova, A.V. Karnaukhov, N.A. Karnaukhova, E.V. Bogdanenko, I.A. Lizunova, V.N. Karnaukhov and Pleiades Publishing, Inc., 2018.

引用

页码：84 / 92

页数：8

共 41 条

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