Development and characterization of 15 microsatellite loci for Cariniana estrellensis and transferability to Cariniana legalis, two endangered tropical tree species

被引：0
作者：
Marcela Corbo Guidugli
Tatiana de Campos
Adna Cristina Barbosa de Sousa
Juliana Massimino Feres
Alexandre Magno Sebbenn
Moacyr Antonio Mestriner
Eucleia Primo Betioli Contel
Ana Lilia Alzate-Marin
机构：
[1] Universidade de São Paulo (USP),Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, Laboratório de Genética Vegetal
[2] Universidade Estadual de Campinas (Unicamp),Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução
[3] Estação Experimental de Tupi,Instituto Florestal de São Paulo
来源：
Conservation Genetics | 2009年 / 10卷
关键词：
Conservation genetics; Forest; Genetic diversity; Jequitibá branco; Molecular marker; SSR;
D O I：
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中图分类号：
学科分类号：
摘要：
From a genomic library enriched for GA/CA repeats, 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis, a tropical forest tree. The microsatellite loci were screened in 49 mature trees found between Pardo river and Mogi-Guaçu river basins, in the state of São Paulo, Brazil. A total of 140 alleles were detected with an average of 9.33 alleles per locus. The expected heterozygosity ranged from 0.37 to 0.88. These loci showed a high probability of paternity exclusion. Additionally, 12 loci were effectively transferred to Cariniana legalis. High levels of polymorphism make the present SSR markers useful for population genetic studies.
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