Fractal analysis of shape changes in murine osteoblasts cultured under simulated microgravity

被引：1
作者：
Fabrizio Testa
Alessandro Palombo
Simona Dinicola
Fabrizio D’Anselmi
Sara Proietti
Alessia Pasqualato
Maria Grazia Masiello
Pierpaolo Coluccia
Alessandra Cucina
Mariano Bizzarri
机构：
[1] University La Sapienza,Department of Experimental Medicine, Systems Biology Group
[2] University Tor Vergata,Systems Biology Group
[3] University La Sapienza,Department of Surgery “Pietro Valdoni”
[4] University G. D’Annunzio,Department of Basic and Applied Medical Science
[5] University La Sapienza,Department of Clinical and Molecular Medicine
来源：
Rendiconti Lincei | 2014年 / 25卷
关键词：
Osteoblast; Fractal dimension; Microgravity; Symmetry breaking; Shape;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Morphological changes have been reported to occur in cells exposed to microgravity, even if quantitative (i.e., fractal) analysis has been never performed on cell shape. We investigated cell shape as well as cytoskeleton modifications induced by simulated microgravity on murine osteoblasts (MC3T3-E1) growing in vitro by means of fractal analysis. On average, after 48–72 h of exposition to microgravity, osteoblasts display significant changes in shape profile, recovering a more rounded phenotype characterized by larger surface area than controls. More specifically, microgravity enacted the emergence of two distinct morphological phenotypes, one of which characterized by an increase in membrane fractal values, surface area, and roundness. Moreover, osteoblasts exposed to microgravity undergo significant functional changes (inhibited growth proliferation, increased apoptosis, β1-integrin decrease, impaired Akt and Erk phosphorylation) that could likely concur in explaining the observed alteration in bone structure experienced by astronauts.
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页码：39 / 47
页数：8
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