Reduced density of intramembranous particle clusters: Freeze-fracture study of mdx mouse muscle plasma membrane

被引：2

作者：

S. Shibuya

Yoshihiro Wakayama

Hiroaki Oniki

机构：

[1] Department of Neurology, Showa University Fujigaoka Hospital, Yokohama 227-8501

[2] Electron Microscope Laboratory, Showa University Fujigaoka Hospital, Yokohama

来源：

Medical Electron Microscopy | 2003年 / 36卷 / 1期

关键词：

Freeze-fracture; Intramembranous particle cluster; Mdx mouse; Muscle plasma membrane; Orthogonal array;

D O I：

10.1007/s007950300008

中图分类号：

学科分类号：

摘要：

Our previous freeze-fracture study demonstrated the decreased density of intramembranous particles (IMPs) on the protoplasmic (P) face of muscle plasma membranes in mdx mice. However, the molecular mechanism is unknown. In the present freeze-fracture study, we examined whether the reduced P-face IMP density in mdx mice would be caused by depletion of the rosette-like IMP clusters, which are IMP aggregations differing from crystal-like orthogonal arrays (OAs). By comparison with control mice, the P-face plasma membranes of extensor digitorum longus (EDL) and soleus (SOL) muscles of mdx mice demonstrated the following findings: (1) decreased IMP density with subunit particles of OAs and IMP clusters, (2) decreased IMP density without subunit particles of OAs, (3) normal IMP density without subunit particles of OAs and IMP clusters, (4) decreased OA density in EDL muscles and normal OA density in SOL muscles, and (5) decreased IMP cluster densities in both muscles. Thus, the reduced IMP density of P-face muscle plasma membranes in mdx mice may result from the decreased IMP clusters, suggesting the relationship between IMP clusters and the integral membrane proteins is influenced by dystrophin deficiency such as that of dystrophin-associated glycoproteins or other membrane proteins.

引用

页码：59 / 65

页数：6

共 23 条

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