Molecular cloning and expression profile analysis of porcine TCAP gene

被引：0
作者：
Hunjun Cheng
Xuewen Xu
Shuhong Zhao
Bang Liu
Mei Yu
Bin Fan
机构：
[1] Huazhong Agricultural University,Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture
来源：
Molecular Biology Reports | 2010年 / 37卷
关键词：
Molecular cloning; Expression profile; SNP; Sarcomere assembly;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The gradually discovered sarcomeric proteins play important roles for structural integrity and signal transduction of sarcomere during myofibril genesis. TCAP (also described as telethonin, T-cap), one of the sarcomeric protein genes, is regulated developmentally. In this study, we reported the molecular characteristics of porcine TCAP gene. A 979 bp TCAP cDNA nucleotide sequence was obtained in pig and the deduced amino acid sequence had 92 and 91% identity to those of human and mouse homologous genes, respectively. One SNP was discovered and the allele frequency analysis showed that G allele frequency was low among 221 unrelated pigs from seven breeds. The tissue distribution patterns revealed that TCAP mRNA was expressed abundantly in skeletal and heart muscle tissue. Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) results displayed TCAP mRNA was up-regulated in both Tongcheng and Landrace pigs during prenatal skeletal muscle development stages. This study suggested that TCAP gene might be a prospective candidate gene affecting muscle mass and meat quality traits in the pig, and also implicated the possible significance of TCAP on sarcomere assembly.
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页码：1641 / 1647
页数：6
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