Secretory peptide PdEPF2 enhances drought tolerance by modulating stomatal density and regulates ABA response in transgenic Arabidopsis thaliana

被引:0
作者
Sha Liu
Congpeng Wang
Fuli Jia
Yi An
Chao Liu
Xinli Xia
Weilun Yin
机构
[1] Beijing Forestry University,National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology
来源
Plant Cell, Tissue and Organ Culture (PCTOC) | 2016年 / 125卷
关键词
Abscisic acid; Drought; Stomatal density; Water use efficiency;
D O I
暂无
中图分类号
学科分类号
摘要
Water deficit limits the growth and productivity of plants worldwide. Improved water use efficiency (WUE) and drought tolerance are important adaptations to address these limitations. In this study, an epidermal patterning factor (EPF), PdEPF2, from a fast-growing poplar clone NE-19 (Populus nigra × (Populus deltoids × Populus nigra)) was isolated. Quantitative reverse transcription polymerase chain reaction showed that transcription of this gene was induced by drought and abscisic acid (ABA). To study the biological functions of PdEPF2, transgenic Arabidopsis plants harboring (35S:PdEPF2) in which PdEPF2 was constitutively expressed were generated. Compared with the wild type and epf2-3 mutant, the transgenic plants ectopically expressing PdEPF2 showed favorable osmotic parameters, such as seed germination rate, primary root length, proline and chlorophyll content, Fv/Fm, photosynthetic rate, and instantaneous leaf WUE, under drought stress. In addition, the transgenic Arabidopsis plants displayed enhanced drought tolerance as a result of decreased stomatal density, which would limit transpiration and reduce water loss. Compared with the wild-type, plants that overexpressed PdEPF2 had decreased sensitivity to exogenous ABA during germination and seedling development, whereas the epf2-3 mutant showed increased sensitivity to ABA. Furthermore, PdEPF2 positively regulated expression of two ABA signaling-related genes, ABI1 and ABI2. These findings indicate that PdEPF2 may enhance drought tolerance by regulating stomatal density and the response to the ABA signaling pathway.
引用
收藏
页码:419 / 431
页数:12
相关论文
共 50 条
[31]   Overexpression of the cassava (Manihot esculenta) PP2C26 gene decreases drought tolerance and abscisic acid responses in transgenic Arabidopsis thaliana [J].
Zeng, Jian ;
Huang, Feifei ;
Zhang, Ning ;
Wu, Chunlai ;
Lin, Jiamiao ;
Lin, Suyan ;
Pan, Zimo ;
Lei, Xinfang ;
Zheng, Jie ;
Guo, Jing ;
Hu, Wei .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2025, 760
[32]   A Novel Sweetpotato WRKY Transcription Factor, IbWRKY2, Positively Regulates Drought and Salt Tolerance in Transgenic Arabidopsis [J].
Zhu, Hong ;
Zhou, Yuanyuan ;
Zhai, Hong ;
He, Shaozhen ;
Zhao, Ning ;
Liu, Qingchang .
BIOMOLECULES, 2020, 10 (04)
[33]   Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB2 Enhances Drought and Salinity Tolerance, Increases Hypersensitivity to ABA and Delays Flowering in Arabidopsis thaliana [J].
Shan, Hong ;
Chen, Sumei ;
Jiang, Jiafu ;
Chen, Fadi ;
Chen, Yu ;
Gu, Chunsun ;
Li, Peiling ;
Song, Aiping ;
Zhu, Xirong ;
Gao, Haishun ;
Zhou, Guoqin ;
Li, Ting ;
Yang, Xue .
MOLECULAR BIOTECHNOLOGY, 2012, 51 (02) :160-173
[34]   A novel rice calmodulin-like gene, OsMSR2, enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis [J].
Guo-Yun Xu ;
Pedro S. C. F. Rocha ;
Man-Ling Wang ;
Meng-Liang Xu ;
Yan-Chun Cui ;
Luo-Ye Li ;
Yu-Xing Zhu ;
Xinjie Xia .
Planta, 2011, 234 :47-59
[35]   Arabidopsis calcineurin B-like-interacting protein kinase 8 and its functional homolog in tomato negatively regulates ABA-mediated stomatal movement and drought tolerance [J].
Liu, Fei ;
Liu, Qi ;
Wu, Ju-Hua ;
Wang, Zong-Qi ;
Geng, Yuan-Jun ;
Li, Juan ;
Zhang, Yan ;
Li, Sha .
PLANT CELL AND ENVIRONMENT, 2024, 47 (07) :2396-2409
[36]   A putative poplar PP2C-encoding gene negatively regulates drought and abscisic acid responses in transgenic Arabidopsis thaliana [J].
Muhammad Arshad ;
Jim Mattsson .
Trees, 2014, 28 :531-543
[37]   Transcription co-activator Arabidopsis ANGUSTIFOLIA3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA (YDA) [J].
Meng, Lai-Sheng ;
Yao, Shun-Qiao .
PLANT BIOTECHNOLOGY JOURNAL, 2015, 13 (07) :893-902
[38]   Arabidopsis thaliana CRK41 negatively regulates salt tolerance via H2O2 and ABA cross-linked networks [J].
Li, Xinyue ;
Zhao, Jun ;
Sun, Yuhui ;
Li, Yingzhang .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2020, 179
[39]   Cotton Late Embryogenesis Abundant (LEA2) Genes Promote Root Growth and Confer Drought Stress Tolerance in Transgenic Arabidopsis thaliana [J].
Magwanga, Richard Odongo ;
Lu, Pu ;
Kirungu, Joy Nyangasi ;
Dong, Qi ;
Hu, Yangguang ;
Zhou, Zhongli ;
Cai, Xiaoyan ;
Wang, Xingxing ;
Hou, Yuqing ;
Wang, Kunbo ;
Liu, Fang .
G3-GENES GENOMES GENETICS, 2018, 8 (08) :2781-2803
[40]   Arabidopsis RING E3 ubiquitin ligase JUL1 participates in ABA-mediated microtubule depolymerization, stomatal closure, and tolerance response to drought stress [J].
Yu, Seong Gwan ;
Kim, Jong Hum ;
Cho, Na Hyun ;
Oh, Tae Rin ;
Kim, Woo Taek .
PLANT JOURNAL, 2020, 103 (02) :824-842