Long-term imaging of dorsal root ganglia in awake behaving mice

被引：0
作者：
Chao Chen
Jinhui Zhang
Linlin Sun
Yiling Zhang
Wen-Biao Gan
Peifu Tang
Guang Yang
机构：
[1] Peking 301 Hospital,Department of Orthopaedics
[2] the Affiliated Southeast Hospital of Xiamen University,Department of Orthopaedics
[3] Columbia University,Department of Anesthesiology
[4] New York University School of Medicine,Department of Neuroscience and Physiology, Department of Anesthesiology, Skirball Institute
来源：
Nature Communications | / 10卷
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摘要：
The dorsal root ganglia (DRG) contain the somas of first-order sensory neurons critical for somatosensation. Due to technical difficulties, DRG neuronal activity in awake behaving animals remains unknown. Here, we develop a method for imaging DRG at cellular and subcellular resolution over weeks in awake mice. The method involves the installation of an intervertebral fusion mount to reduce spinal movement, and the implantation of a vertebral glass window without interfering animals’ motor and sensory functions. In vivo two-photon calcium imaging shows that DRG neuronal activity is higher in awake than anesthetized animals. Immediately after plantar formalin injection, DRG neuronal activity increases substantially and this activity upsurge correlates with animals’ phasic pain behavior. Repeated imaging of DRG over 5 weeks after formalin injection reveals persistent neuronal hyperactivity associated with ongoing pain. The method described here provides an important means for in vivo studies of DRG functions in sensory perception and disorders.
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