Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

被引：15

作者：

Abdullah A.A. ^{[1
]}

Ismail M.F.B. ^{[2
]}

Balakrishnan K.N. ^{[2
]}

Bala J.A. ^{[2
]}

Hani H. ^{[2
]}

Abba Y. ^{[2
]}

Awang Isa M.K. ^{[2
]}

Abdullah F.F.J. ^{[3
]}

Arshad S.S. ^{[2
]}

Nazariah Z.A. ^{[1
,2
]}

Abdullah R. ^{[1
,2
]}

Mustapha N.M. ^{[2
]}

Mohd-Lila M.-A. ^{[1
]}

机构：

[1] Institute of Bioscience, University Putra Malaysia, Serdang, 43400, Selangor DE

[2] Department of Pathology and Microbiology, Universiti Putra Malaysia, Serdang, 43400, Selangor DE

[3] Department of Veterinary Clinical Studies, Universiti Putra Malaysia, Serdang, 43400, Selangor DE

来源：

VirusDisease | 2015年 / 26卷 / 4期

关键词：

Contagious ecthyma; Goat; Orf virus; Parapoxvirus; Sore-mouth infection;

D O I：

10.1007/s13337-015-0278-4

中图分类号：

学科分类号：

摘要：

Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally. © 2015, Indian Virological Society.

引用

页码：255 / 259

页数：4

共 28 条

[1]

Abrahao J.S., Campos R.K., Trindade G.S., Guedes M.I., Lobato Z.I., Mazur C., Et al., Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report, Virol J, 6, (2009)

[2]

AL-Ajeeli K.S.A., Studies O.N., Malaysian isolates of Orf virus, (1995)

[3]

Bande F., Suriarshad S., Abdullah F.F.J., Mohd Saleh A.S., Molecular characterization of Malaysian Orf virus from outbreak cases in Goats, 26th Veterinary Association congress, vol, (2014)

[4]

Bora M., Bora D., Barman N., Borah B., Bora P., Talukdar A., Et al., Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam, VirusDisease, 26, 1-2, pp. 82-88, (2015)

[5]

Chi X., Zeng X., Hao W., Li M., Li W., Huang X., Et al., Heterogeneity among Orf virus isolates from goats in Fujian Province, Southern China, PLoS ONE, 8, 10, (2013)

[6]

Essbauer S., Pfeffer M., Meyer H., Zoonotic poxviruses, Vet Microbiol, 140, 3-4, pp. 229-236, (2010)

[7]

Fleming S.B., Mercer A.A., Genus parapoxvirus, Birkhäuser advances in infectious diseases. Poxviruses, pp. 127-165, (2007)

[8]

Georgiades G., Katsarou A., Dimitroglou K., Human ORF (ecthyma contagiosum), J Hand Surg Br, 30, 4, pp. 409-411, (2005)

[9]

Guo J., Rasmussen J., Wunschmann A., de La Concha-Bermejillo A., Genetic characterization of Orf viruses isolated from various ruminant species of a zoo, Vet Microbiol, 99, 2, pp. 81-92, (2004)

[10]

Hagis A.M., Ginn P.E., Integumentary system, Thomson’s special veterinary pathology, (2001)

← 1 2 3 →