Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glycopyranosyl isothiocyanate

被引：0
作者：
Kyeong Ho Kim
Tae Kyun Kim
机构：
[1] Kangwon National University,College of Pharmacy
[2] Choseon Pharm. & Trading Co.,Institute of Life Science Research
来源：
Archives of Pharmacal Research | 1998年 / 21卷
关键词：
Chiral separation; HPLC; Chiral derivatizing reagent; Salbutamol; Enantiomer; 2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate;
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摘要：
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1. 83) on 5 μm octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3).
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页码：217 / 222
页数：5
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