Identification of the bioactive core component of the insecticidal Vip3A toxin peptide of Bacillus thuringiensis

被引：0
作者：
Srimonta Gayen
Munshi Azad Hossain
Soumitra K. Sen
机构：
[1] University of Michigan,Department of Human Genetics
[2] University of California,Molecular Biology Section, Division of Biology
[3] San Diego,Advanced Laboratory for Plant Genetic Engineering
[4] Indian Institute of Technology,undefined
来源：
Journal of Plant Biochemistry and Biotechnology | 2012年 / 21卷
关键词：
Vegetative insecticidal protein; toxin; Bioactive core component; Transgenic insect resistant plant; Enhanced insecticidal potency; N-terminus deletion mutant;
D O I：
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中图分类号：
学科分类号：
摘要：
The potential of insecticidal Vip3Aa toxin peptide of Bacillus thuringiensis (Bt) as a resource for development of lepidopteran insect resistant transgenic crop plants has not yet been fully fathomed. The single mode of protection offered by the insecticidal Vip3Aa toxin against a broad spectrum of lepidopteran insect pests that invade crop field as secondary insect pests, carry definitive significance. However, lack of diversity amongst insecticidal Vip3A toxin towards toxicity for lepidopteran insects is often considered as disadvantage. In order to bring in improvement at this front, search for diversity and protein engineering of the toxin molecule for creation of diversity require to be undertaken in future. In that context, identification of the bioactive core component of Vip3BR toxin peptide of Bt an analogue of Vip3Aa toxin has been accomplished. The core component was found to contain enhanced potency of the insecticidal property 2–3 folds more than the native toxin against four major crop pests.
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页码：128 / 135
页数：7
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