Involvement of ERK1/2 and p38 MAPK in up-regulation of 14-3-3 protein induced by hydrogen peroxide preconditioning in PC12 cells

被引：9
作者：
Su Q.-J. [1 ,2 ]
Chen X.-W. [2 ]
Chen Z.-B. [2 ]
Sun S.-G. [1 ]
机构：
[1] Department of Neurology, Huazhong University of Science and Techology
[2] Department of Neurology, Hainan Medical College
来源：
Neuroscience Bulletin | 2008年 / 24卷 / 4期
基金：
中国国家自然科学基金;
关键词：
14-3-3; protein; ERK1/2; Hydrogen peroxide preconditioning; P38 mitogen-activated protein kinase; PC12; cell;
D O I：
10.1007/s12264-008-0307-z
中图分类号：
学科分类号：
摘要：
Objective: To investigate the protective effects of hydrogen peroxide preconditioning (HPP) on the pheochromocytoma (PC12) cells treated with 1-methyl-4-phenylpyridinium (MPP+) and to explore the potential mechanisms. Methods: The viability and apoptosis of PC12 cells were determinded by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 4′,6′-diamidino-2-phenylindole (DAPI) staining, respectively. The expressions of 14-3-3 protein and phospholylated p38 mitogen-activated protein kinase (MAPK) were determined by Western blot. Enzyme-linked immunosorbent assay (ELISA) was used to measure the activity of extracellular signal-regulated protein kinase 1/2 (ERK1/2). Results: The cell viability decreased and the number of apoptotic cells increased dramatically in MPP+ group compared with that in Control group. HPP induced a significant increase in cell viability and a marked decrease in population of apoptotic cells of the MPP +-treated PC12 cells, accompanied with up-regulation of 14-3-3 protein and increase of ERK1/2 and p38 MAPK activities. The 14-3-3 protein expression was positively correlated with the phosphorylation of ERK1/2. Furthermore, inhibition of the ERK1/2 with PD98059 abolished the 14-3-3 protein up-regulation in PC12 cells induced by HPP. Conclusion: HPP protects PC12 cells against MPP+ toxicity by up-regulating 14-3-3 protein expression through the ERK1/2 and p38 MAPK signaling pathways. © 2008 Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag GmbH.
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页码：244 / 250
页数：6
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