Simultaneous Detection of C282Y and H63D Hemochromatosis Mutations by Dual-color Probes

被引：0
作者：
Marec Phillips
Cindy A. Meadows
Ming Y. Huang
Alison Millson
Elaine Lyon
机构：
[1] Associated and Regional University Pathologist Institute for Clinical and Experimental Pathology,Department of Pathology
[2] Associated and Regional University Pathologist Laboratories,undefined
[3] University of Utah School of Medicine,undefined
[4] ARUP Laboratories,undefined
来源：
Molecular Diagnosis | 2000年 / 5卷 / 2期
关键词：
mutation detection; melting curve analysis; fluorescent hybridization probes;
D O I：
10.1007/BF03262029
中图分类号：
学科分类号：
摘要：
Background: Hemochromatosis is a common genetic disease, affecting one in every 200 individuals in the United States. A PCR assay was designed using fluorescent melting curve analysis to simultaneously detect the G845→A (C282Y) and C187→G (H63D) mutations. The G845→A and C187→G loci are distinguished by color, and mutant alleles are distinguished from wild type by probe melting temperature (Tm).
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页码：107 / 116
页数：9
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