Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

被引：9

作者：

Chan K.-W. ^{[1
]}

机构：

[1] Research and Instrumentation Unit, Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI), Petaling Jaya

来源：

Egyptian Journal of Forensic Sciences | / 7卷 / 1期

关键词：

HPLC; Illicit Heroin; Morphine; Quantitative method;

D O I：

10.1186/s41935-017-0003-0

中图分类号：

学科分类号：

摘要：

Background: Morphine is illegal to use unless it is prescribed by a medical doctor. This compound is commonly abused in the form of illicit heroin. Methods: An easy-to-use method for determining morphine in illicit heroin using high performance liquid chromatography-photodiode array detector (HPLC-PDA) was developed. With the aid of 50:50 acetonitrile:ammonium formate, this target compound traveling in a pentafluorophenyl (PFP) column was successfully separated from other compounds frequently present in the heroin matrix. Results: The method was precise with an intra-day relative standard deviation (RSD) ≤ 0.8% and inter-day RSD < 5%. It was able to detect as low as 0.001 mg/mL morphine. The detector’s response was linear (R2 > 0.999) and reliable for morphine quantitation from 0.005 up to 1 mg/mL. Through recovery studies, accuracy of the method was averagely estimated to be 89.54–101.91%. Conclusions: This method saves time in terms of mobile phase preparation and cuts cost by excluding additional purchase of expensive chemicals. More importantly, it proves to be able to determine the target analyte with sufficient accuracy and precision. © 2017, The Author(s).

引用

共 24 条

[1]

Baker P.B., Gough T.A., The separation and quantitation of the narcotic components of illicit heroin using reversed-phase high performance liquid chromatography, J Chromatogr Sci, 19, pp. 483-489, (1981)

[2]

Barnfield C., Burns S., Byrom D.L., Kemmenoe A.V., The routine profiling of forensic heroin samples, Forensic Sci Int, 39, pp. 107-117, (1988)

[3]

Chan K.W., Tan G.H., Wong R.C.S., Gas chromatographic method validation for the analysis of major components in illicit heroin seized in Malaysia, Sci Justice, 52, pp. 9-16, (2012)

[4]

Cole M.D., Caddy B., The analysis of drugs of abuse: an instructional manual, (1995)

[5]

Gough T.A., Baker P.B., The selection of gas chromatographic stationary phases and operating conditions for the separation and quantitation of heroin and structurally related compounds, J Chromatogr Sci, 19, pp. 227-234, (1981)

[6]

Kaa E., Bent K., Impurities, adulterants and diluents of illicit heroin in Denmark (Jutland and Funen), Forensic Sci Int, 31, pp. 195-210, (1986)

[7]

Klous M.G., Lee W.C., Hillebrand M.J.X., Brink W., Ree J.M., Beijnen J.H., Analysis of diacetylmorphine, caffeine, and degradation products after volatilation of pharmaceutical heroin for inhalation, J Anal Toxicol, 30, pp. 6-13, (2006)

[8]

Lee D.K.T., Chiou A.H.J., Wang D.P., Simultaneous determination of morphine HCl, ketamine HCl and droperidol in 0.9% sodium chloride by HPLC, J Food Drug Anal, 13, 1, pp. 93-95, (2005)

[9]

Lurie I.S., Carr S.M., Heroin and selected basic impurities via reversed phase HPLC. I.: analysis of unadulterated heroin samples, J Liq Chromatogr, 9, pp. 2485-2509, (1986)

[10]

Moros J., Galipienso N., Vilches R., Garrigues S., Guardia M.D.L., Nondestructive direct determination of heroin in seized illicit street drugs by diffuse reflectance near-infrared spectroscopy, Anal Chem, 80, pp. 7257-7265, (2008)

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