Pre-termination in aflR of Aspergillus sojae inhibits aflatoxin biosynthesis

被引:0
作者
K. Matsushima
P.-K. Chang
J. Yu
K. Abe
D. Bhatnagar
T. Cleveland
机构
[1] Research and Development Division,
[2] Kikkoman Corporation,undefined
[3] 399 Noda,undefined
[4] Noda-City,undefined
[5] Chiba 278–0037,undefined
[6] Japan,undefined
[7] Southern Regional Research Center,undefined
[8] Agricultural Research Service,undefined
[9] U.S. Department of Agriculture,undefined
[10] 1100 Robert E. Lee Boulevard,undefined
[11] New Orleans,undefined
[12] LA 70124,undefined
[13] USA,undefined
[14] Present address: Graduate School of Agricultural Sciences,undefined
[15] Tohoku University,undefined
[16] 1–1 Amamiya,undefined
[17] Tsutsumi-dori,undefined
[18] Aoba-ku,undefined
[19] Sendai 981–8555,undefined
[20] Japan,undefined
来源
Applied Microbiology and Biotechnology | 2001年 / 55卷
关键词
Codon; Gene Product; Aspergillus; Transcriptional Regulator; Aflatoxin;
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学科分类号
摘要
The aflR gene product is the main transcriptional regulator of aflatoxin biosynthesis in Aspergillus parasiticus and Aspergillus flavus. Although A. sojae strains do not produce aflatoxins, they do have an aflR homologue. When compared with the aflR of A. parasiticus, the A. sojae gene contains two mutations: an HAHA motif and a premature stop codon. To investigate the functionality of the A. sojae aflR gene product, we used a GAL4 one-hybrid system in yeast. The transcription-activating activity of AflR from A. sojae was 15% of that from A. parasiticus. The introduction of an additional aflR from A. sojae into an A. parasiticus strain did not affect aflatoxin productivity. A hybrid aflR comprising the amino-terminal region of A. sojae aflR and the carboxy-terminal region of A. parasiticus aflR suppressed the effect associated with pre-termination of the A. sojae AflR. We conclude that the premature stop codon of the A. sojae aflR is the key to its functionality and leads to prevention of aflatoxin biosynthesis through loss of the transcription of aflatoxin biosynthesis-related genes.
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页码:585 / 589
页数:4
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